Human Epidermal Keratinocytes, Pooled (HEKp)

Catalog number:  A13401

Gibco™  Related applications: Primary Cell Culture

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Human Epidermal Keratinocytes, pooled (HEKp) from Invitrogen are normal cells isolated from multiple neonatal foreskins. These cells are optimal for investigating the molecular and biochemical bases of normal and disease processes of the skin. They have been cryopreserved at the end of the primary culture stage in animal origin-free media containing 10% DMSO. Use HEKp for your research into fibrosis, wound healing, irritancy, and diseases such as psoriasis.
Guaranteed to have ≥70% viability upon thawing
• Each vial guaranteed to reach ≥15 population doublings after thaw
• Sufficient cells to seed ≥100 cm2
• Tested for potential biological contaminants
• Optimized for use in EpiLife® serum-free media

Get More Physiologically Relevant Results with Invitrogen Primary Cells

Human primary cell cultures closely mimic the in vivo state and thus generate more physiologically relevant data. Our Human Epidermal Keratinocytes are isolated and pooled from 4-6 neonatal foreskins. This process is robust and yields large donor lots to ensure availability of consistent product for your studies.

Reliable Performance and Purity
Primary cells from Invitrogen are guaranteed to have ≥70% viability upon thawing with each vial containing sufficient cells to seed ≥100 cm2 of surface area. Performance testing guarantees each vial of HEKp will reach ≥15 population doublings after thaw. In addition, each lot is tested for potential biological contaminants--HIV-1, Hepatitis B, Hepatitis C viruses, mycoplasma, bacteria, yeast, and other fungi-- to ensure quality and robustness.

Optimized for Use with Gibco® Media
Human Epidermal Keratinocytes, pooled, from Invitrogen have been optimized for use with our serum-free media, specifically EpiLife® Medium with 60 µM calcium and Human Keratinocyte Growth Supplement (HKGS). Preparation of supplemented media is a simple process; the final solution can be stored for up to 30 days at 4°C.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Learn more about our complete system of media and supplements for culturing Human Epidermal Keratinocytes
For Research Use Only. Not for use in diagnostic procedures.


LoyaltyEligible: SKUEligibleForLoyalty
Age: Newborn (14 days or less)
Cell Passage Number: Primary culture stage
Cell Type: Keratinocytes
Classification: Serum-Free
Culture Type: Adherent Cell Culture
Donor Attributes: Normal
Donor Source: Pooled
Form: Cryopreserved
Format: Vial(s)
Gender: Male
Number of Cells: ≥ 1 x 106
Product Line: Gibco®
Product Size: 1 vial
Species: Human
Tests Performed: Growth Performance Assay, HIV-1, Hepatitis B (HBV), Hepatitis C (HCV), Mycoplasma, Sterility Testing (Bacteria & Fungi)

Contents & storage

≥ 1 × 106 viable cells


Manuals & protocols