CytoTune®-iPS Sendai Reprogramming Kit - FAQs

The Invitrogen CytoTune-iPS 2.0 Kit (Cat. Nos. A16517, A16518) offers higher reprogramming efficiency, faster clearance of the vectors, and lower cytotoxicity compared to the original Invitrogen CytoTune-iPS Sendai Reprogramming Kit (Cat. Nos. A13780-01, A13780-02) that has been discontinued.

The 2.0 kit contains three vectors, one of which is a polycistronic vector (Invitrogen CytoTune 2.0 KOS), designed to deliver increased reprogramming efficiency. This polycistronic vector has a different backbone containing temperature-sensitive mutations in the polymerase-related genes, and this helps to clear the virus faster after reprogramming and causes less cytotoxicity to the cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The Invitrogen CytoTune -iPS Sendai Reprogramming Kit (Cat. Nos. A13780-01, A13780-02) is a non-integrating system that reprograms somatic cells into induced pluripotent stem cells (iPSCs). This kit utilizes four Sendai virus-based vectors, each capable of expressing one of the four Yamanaka factors: Oct3/4, Sox2, Klf4, and c-Myc. The expression of these transcription factors in somatic cells has been shown to be a critical factor in the successful generation of iPSCs.

Please note that the Invitrogen CytoTune-iPS Sendai Reprogramming Kit (Cat. Nos. A13780-01, A13780-02) has been discontinued and replaced by the Invitrogen CytoTune-iPS 2.0 Sendai Reprogramming Kit (Cat. Nos. A16517, A16518).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.