StemPro® CD34+ Cell Kit

Catalog number: A14059

 Related applications: Stem Cell Culture | Stem Cell Engineering & Reprogramming

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Description

StemPro® CD34+ cells are pooled Human Hematopoietic Progenitor cells (HPCs) derived from the umbilical cord blood of mixed donors. Along with 0.5×106 pooled donor cord blood-derived CD34+ cells, this kit includes StemPro®-34 SFM basal liquid medium and frozen StemPro®-34 Nutrient Supplement to facilitate the immediate culturing of the StemPro® CD34+ cells upon arrival or when recovering from cryopreservation storage.

With the StemPro® CD34+ Cell Kit You Get:
• ≥90% purity of the desired CD34+ cell population
• Each vial of cells contains 0.5×106 CD34+ cells with full traceability
• StemPro®-34 SFM manufactured following cGMP and ISO13485
• High reprogramming efficiency to iPSCs
Custom sizes available upon request

Included in this kit are:
• One vial containing one ml of 0.5×106 cells/ml pooled donor cord blood-derived CD34+ cells
• 500 ml StemPro®-34 SFM basal liquid medium
• 13 ml frozen StemPro®-34 Nutrient Supplement

Hematopoietic Progenitor Cells
Hematopoietic progenitor cells (HPCs) or hematopoietic stem cells (HSCs) can be found in the blood, bone marrow, umbilical cord blood, or derived from Embryonic Stem Cells (ESCs) or induced pluripotent stem cells (iPSCs). They are multipotent stem cells capable of forming every cell lineage in the blood and immune system. Hematopoietic progenitor cells (HPCs) are non-adherent, with a small, round morphology. A fundamental property of these cells is their capacity to self-renew, thus providing, in principle, a limitless source of daughter cells with the same functional properties. Several cell surface markers used for identifying human HPCs/HSCs include CD34+, CD59+, Thy1/CD90+, CD38 lo/-, C-kit/CD117+, Lin-.

Common Applications
Hematopoietic stem cells (HSCs) are most commonly used in transplantation therapies to treat illnesses such as leukemia, lymphoma, and sickle cell diseases by reconstituting a patient's hematopoietic cell system.

High Purity Stem Cells for Higher Reprogramming Efficiency
HPCs/HSCs can be reprogrammed to induced pluripotent stem cells (iPSCs) for use as disease models in stem cell research. StemPro® CD34+ cells have ≥90% CD34+ cells, as determined by Flow Cytometry, affording higher reprogramming efficiency than less pure populations. Protocols using CytoTune®-iPS Sendai Reprogramming Kit are available on our website and included in the product insert.

Isolation of CD34+ Cells from Cord Blood
StemPro® CD34+ primary cord blood cells from multiple human donors originating and collected in the United States are pooled and then enriched for the CD34+ cell population using immunomagnetic CD34 MicroBeads. Pooling the cord blood from multiple donors reduces the lot-to-lot variability of the product. The cells are derived from tissue obtained from accredited institutions through an IRB (Institutional Review Board) approved donor program and are negative for HIV and Hepatitis B.

Quality control
QC for the StemPro® CD34+ cells includes testing for the following: mycoplasma, sterility, endotoxin, and FACS profile.

QC for StemPro-34 SFM includes testing for the following: Sterility, pH, endotoxin, osmolality, Hepatitis B Surface Antigen, HIV 1&2 antibody, HCV antibody, and CD34+ cell performance assay.

Serum Free Medium Developed for CD34+ Cell Expansion
StemPro®-34 SFM is comprised of a basal liquid medium and frozen StemPro®-34 Nutrient Supplement. This serum-free media system was developed to support the growth of human CD34+ hematopoietic progenitor cells. Sera can vary from lot to lot in its ability to support cell-specific growth in culture. Serum-free culture systems eliminate this major variable in the study of hematopoiesis ex vivo. StemPro®-34 SFM can be purchased separately.

See a list of selected publications citing the Sendai virus for iPSC generation here.

For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Age: Newborn (14 days or less)
Cell Type: Stem Cells (Hematopoietic)
Classification: Serum-Free
Culture Type: Suspension Cell Culture
Donor Attributes: Normal
Form: Cryopreserved
Media Recommendation: StemPro®-34 SFM Media
Number of Cells: 0.5 x 106
Product Size: 0.5 x 106 cells⁄mL
Quantity: 1 kit
Species: Human

Contents & storage

Storage of StemPro® CD34+ Cells: Store in liquid nitrogen

Storage of StemPro®-34 SFM Media: Store at 2° to 8° C

Storage of StemPro 34 supplement: Store at -5° to -20° C

Documents

Manuals & protocols

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