BacMam Histone H3K9me2 Cellular Assay Kit

Catalog number: A14161

Invitrogen™  Related applications: Target & Lead Identification & Validation

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The BacMam Histone H3K9me2 Cellular Assay Kit is a high-throughput screening (HTS)-compatible TR-FRET assay for the interrogation of dimethylation at lysine 9 on histone H3 in a cellular format. Baculovirus-mediated gene delivery (BacMam) provides a convenient tool for the expression of GFP-histone H3 fusion protein in your cell background of interest. Coupled with a terbium (Tb)-labeled site-specific antibody, the resulting cellular assay can be used to identify inhibitors of methyltransferases and demethylases acting on lysine 9 of histone H3.

The kit includes:
• BacMam Histone H3 Reagent
• LanthaScreen® Tb-anti-histone H3K9me2 Antibody
• 6X LanthaScreen® Cellular Lysis Buffer
• Instrument Control Terbium TR-FRET Kit

With the BacMam Histone H3K9me2 Cellular Assay Kit you can:
• Use your cell background of choice with the portability of BacMam
• Identify more relevant inhibitors since the methyl transferases and demethylases are in their natural protein complexes
• Conserve precious cell samples with a homogenous assay format that can be easily miniaturized
• Improve data quality with the advantages of TR-FRET

Get More Physiologically Relevant Results
The BacMam Histone H3K9me2 Cellular Assay Kit allows the investigation of dimethylation at lysine 9 on histone H3 with your choice of cellular backgrounds, including primary cells. This enables screening for potential inhibitors of methyl transferases and demethylases associated with Lys9 dimethylation in their natural complexes in a physiologically relevant cell type.

BacMam and LanthaScreen® Convenience Saves Sample and Time
Assays are run in a fully homogenous, addition-only format without any of the washing, lysate transfer, or separation procedures required for traditional methods such as western blotting and ELISA, making them ideal for HTS applications. By using the same BacMam reagent for GFP-histone H3, other histone H3 modifications (Ph, Me, Ac) can be monitored by simply exchanging the Terbium (Tb)-labeled antibody. In addition, the application of the LanthaScreen® technology includes all the advantages of TR-FRET detection including reduced data noise, less interference from fluorescent compounds, and high sensitivity, which results in the need to use fewer cells than traditional western or ELISA methods.

For research use only. Not for human or animal therapeutic or diagnostic use.

Learn more about cellular epigenetics assays

Specifications

System: LanthaScreen®
Reporter: GFP (EmGFP)
Technique: TR-FRET
Cell State: Cells Not Included
Product Size: 1 kit
Delivery Method: Baculovirus (BacMam System)
Detection Method: Fluorescent
Druggable Target: Demethylases, Methyltransferases

Contents & storage

The kit includes:
• BacMam Histone H3 Reagent
• LanthaScreen® Tb-anti-histone H3K9me2 Antibody
• 6X LanthaScreen® Cellular Lysis Buffer
• Instrument Control Terbium TR-FRET kit

Upon receipt, store the BacMam Histone H3 Reagent, 6X LanthaScreen® Cellular Lysis Buffer, and Instrument Control Terbium TR-FRET kit at 4°C and the LanthaScreen® Tb-anti-histone H3K9me2 Antibody at -20°C.

Documents

Manuals & protocols

Recommended products