LanthaScreen® Tb-anti-Histone H3K4me3 Antibody

Catalog number: A14167

 Related applications: Chromatin Biology | Target & Lead Identification & Validation

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Description

The LanthaScreen® Tb-anti-Histone H3K4me3 Antibody is a key component of the BacMam Histone H3K4me3 Cellular Assay (A14163). When this terbium (Tb)- labeled antibody is paired with baculovirus-mediated (BacMam) expression of a Histone H3-GFP fusion protein, the TR-FRET readout enables sensitive detection of H3 lysine 4 trimethylation. The resulting high-throughput screening (HTS) compatible assay can be used to identify inhibitors of methyltransferases and demethylases acting on lysine 4 of histone H3 in the cell type of your choice.

LanthaScreen® Tb-anti-Histone H3K4me3 Antibody Lets You:

• Identify more relevant inhibitors since the methyl transferases and demethylases are in their natural protein complexes
• Conserve precious cell samples with the miniaturizable homogenous assay format
• Improve data quality with the advantages of TR-FRET

Get More Physiologically Relevant Results
The LanthaScreen® Histone H3K4me3 antibody allows investigation of trimethylation at lysine 4 on histone H3. This enables screening for potential inhibitors of methyl transferases and demethylases associated with Lys4 trimethylation in their natural complexes in a physiologically relevant cell type. Please note that the LanthaScreen® Tb-anti-Histone H3K4me3 antibody has been shown to have higher affinity for trimethylation compared to dimethylation at lysine 4 using a histone H3 peptide; however, when interpreting your results it is important to consider that the antibody does show some affinity for the dimethylated peptide. Further discrimination between di- and trimethylation can be accomplished by using our complimentary LanthaScreen® Tb-anti-Histone H3K4me2 Antibody (Cat # A14158 and A14153), which is specific for dimethylation. For more information on the specificity of the antibody please see the application note for the BacMam Histone H3K4me3 Cellular Assay Kit (Cat No A14163).

BacMam and LanthaScreen® Convenience Saves Samples and Time
Assays are run in a fully homogenous, addition-only format without any of the washing, lysate transfer, or separation procedures required for traditional methods such as Western blotting and ELISA, making it ideal for HTS applications. By using the same BacMam reagent for GFP-Histone H3, other histone H3 modifications (Ph, Me, Ac) can be monitored just by exchanging the Terbium (Tb) labeled antibody. In addition, the application of the LanthaScreen® technology includes all of the advantages of TR-FRET detection including reduced data noise, less interference from fluorescent compounds, and high sensitivity, allowing the use of fewer cells than traditional Western or ELISA methods.

For research use only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Label or Dye: Tb (Terbium)
Product Size: 1 mg
Concentration: 0.5 mg⁄ml, 3500 nM
Target Modification State: Methylated
Shipping Condition: Dry Ice

Contents & storage

Store the LanthaScreen® Tb-anti-histone H3K4me3 Antibody at -20° C.

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