RiboMinus™ Eukaryote Kit v2
RiboMinus™ Eukaryote Kit v2
Invitrogen™

RiboMinus™ Eukaryote Kit v2

The RiboMinus™ Eukaryote Kit v2 provides truly complete transcriptome isolation for next generation sequencing analysis by offering selective depletion ofRead more
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Catalog NumberQuantity
A1502012 reactions
Catalog number A15020
Price (USD)
1,319.65
Online Exclusive
1,380.00
Save 60.35 (4%)
Each
Add to cart
Quantity:
12 reactions
Price (USD)
1,319.65
Online Exclusive
1,380.00
Save 60.35 (4%)
Each
Add to cart

The RiboMinus™ Eukaryote Kit v2 provides truly complete transcriptome isolation for next generation sequencing analysis by offering selective depletion of up to 99.9% of the 5S, 5.8S, 18S, and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which introduce bias and select for only part of the transcriptome.

Features of the kit include:

• Seamless integration with RiboMinus™Concentration Module (available separately) to purify RNA after ribosomal RNA depletion
• 1.5-hour workflow
• Input range of 1-5 μg

The RiboMinus™ Eukaryote Kit v2 improves upon the previous system (RiboMinus™ Eukaryote Kit for RNA-Seq) by removing significantly more rRNA (both cytoplasmic and mitochondrial) from the total RNA. When used with our library construction kits for sequencing, the RiboMinus™ Eukaryote Kit v2 provides maximum mapping to reference database with minimum bias, maintaining relative gene expression levels of even smaller RNA species. The resulting total RNA after depletion contains minimum contaminating rRNA with reduced variability.

The RiboMinus Eukaryote Kit v2 is designed for the removal of human, mouse, and rat rRNA. The RiboMinus™ Concentration Module (available separately) is used for RNA purification after rRNA depletion. For magnetic bead-based purification, please see the RiboMinus™ Eukaryote System v2. The RiboMinus Eukaryote Kit v2 has an RNA input range of 1-5 μg. For lower inputs (down to 100 ng), the Low Input RiboMinus™ Eukaryote System v2 is recommended.

The RiboMinus™ Eukaryote Kit v2 contains sufficient reagents to perform 12 ribosomal RNA depletions.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypeTranscriptome RNA
For Use With (Equipment)Ion PGM™ System, Ion Proton™ System, Illumina™ Genome Analyzer II (GA II)
Product LineRiboMinus™
Quantity12 reactions
Sequencing TypeTranscriptome Sequencing
Starting Material Amount1 - 5 μg total RNA
Workflow StepSample Enrichment
Isolation TechnologyMagnetic Bead
SpeciesHuman, Mouse, Rat
Unit SizeEach
Contents & Storage

• RiboMinus™ Eukaryote Probe Mix, 2-20°C
• RiboMinus™ Magnetic Beads, 4°C
• 2 x Hybridization Buffer, room temperature