PolarScreen™ Progesterone Receptor Competitor Assay Kit, Green

Catalog number: A15905

 Related applications: Target & Lead Identification & Validation

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Description

The PolarScreen™ Progesterone Receptor Competitor Assay Kit, Green, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential progesterone receptor (PR) ligands. The kit uses insect cell-expressed, human PR ligand binding domain tagged with glutathione-S-transferase (GST) (also available separately) and a novel, high-affinity, fluorescent progesterone receptor ligand (Fluormone™ PL Green) in a homogenous, mix-and-read assay format.

This kit produces a green color read-out; a "red" kit is also available. Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation. For additional information or assistance contact us at drugdiscoverytech@lifetech.com.

The PolarScreen Progesterone Receptor Competitor Assay is:

• Complete & ready-to-use out of the box—all reagents included, just add your test compounds
• High throughput compatible—fluorescence polarization assays in a 384-well plate
• Convenient—components available in bulk sizes for larger screening needs

How the Assay Works
An N-terminal fusion of glutathione transferase to the ligand-binding domain of the human progesterone receptor (PR-LBD(GST)) is added to a fluorescently-tagged progesterone ligand (Fluormone PL Green) in the presence of competitor test compounds in microwell plates. The presence of effective competitors prevents the formation of a PL Green/PR-LBD(GST) complex, resulting in a decrease of the polarization value. The shift in polarization value in the presence of test compounds is used to determine relative affinity of test compounds for PR-LBD(GST).

Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 400 x 20 µL assays
Validated Application: Competitive Binding Assay, Nuclear Receptor Assay

Contents & storage

1 tube Progesterone Receptor Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)
1 tube of Fluormone PL Green (store at -5 to -30°C)
2 bottles of PR Screening Buffer (store at -5 to -30°C)
1 tube of 1 M DTT (store at -5 to -30°C)

Documents

Manuals & protocols

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