Eosin B
Eosin B
Eosin B
Thermo Scientific Chemicals

Eosin B

CAS: 548-24-3 | C20H6Br2N2Na2O9
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Catalog number A17377.14
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Chemical Identifiers
CAS548-24-3
IUPAC Namedisodium 2-(4,5-dibromo-2,7-dinitro-6-oxido-3-oxo-3H-xanthen-9-yl)benzoate
Molecular FormulaC20H6Br2N2Na2O9
InChI KeyGYYTYUGGVBYJHE-UHFFFAOYSA-L
SMILES[Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(C(=O)C(Br)=C2OC2=C(Br)C([O-])=C(C=C12)[N+]([O-])=O)[N+]([O-])=O
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SpecificationsSpecification SheetSpecification Sheet
Appearance (Color)Dark green to red/brown or dark brown
FormPowder
Loss on Drying≤10.0% (105°C/constant wt)
lambda-max (UV-Vis)515-525nm ( in water)
Specific Absorbance (E 1%/1cm)>700 (@ lambda max)
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Eosin B sodium salt is a cell and tissue stain. Eosin B sodium salt is used as a counter stain for collagen, as a tissue stain for cell granules, nuclei, and microorganisms when coupled with Azure A (sc-203729), and as a stain for cell differentiation of the anterior pituitary.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Eosin B sodium salt is a cell and tissue stain. Eosin B sodium salt is used as a counter stain for collagen, as a tissue stain for cell granules, nuclei, and microorganisms when coupled with Azure A (sc-203729), and as a stain for cell differentiation of the anterior pituitary.

Solubility
Soluble in water (390 g/L at 20°C), and ethanol.

Notes
Store at room temperature. Keep away from strong oxidizing agents.
RUO – Research Use Only

General References:

  1. G G Glenner.; R D Lillie. A rhodocyan technic for staining the anterior pituitary.Stain technology. 1957, 32 (4),187-190.
  2. F. Vera.; R. Schrebler.; E. Muñoz.; C. Suarez.; P. Cury.; H. Gómez.; R. Córdova.; R.E. Marotti.; E.A. Dalchiele. Preparation and characterization of Eosin B- and Erythrosin J-sensitized nanostructured NiO thin film photocathodes. Thin Solid Films. 2005, 490 (2),182-188.