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FluoroBrite™ DMEM
Zitierungen und Referenzen (21)
Gibco™

FluoroBrite™ DMEM

Gibco™ FluoroBrite™ DMEM verfügt über eine Hintergrund-Fluoreszenz, vergleichbar mit der von PBS und 90 % niedriger als bei standardmäßigem phenolrot-freiemWeitere Informationen
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KatalognummerMenge
A1896701500 mL
A189670210 x 500 mL
Katalognummer A1896701
Preis (EUR)
48,39
Each
Menge:
500 mL
Preis (EUR)
48,39
Each
Gibco™ FluoroBrite™ DMEM verfügt über eine Hintergrund-Fluoreszenz, vergleichbar mit der von PBS und 90 % niedriger als bei standardmäßigem phenolrot-freiem DMEM. Die Rezeptur umfasst die erforderlichen Nährstoffe für routinemäßige Zellkulturen, ergänzt mit 10 % Rinderfötenserum und 4 mM L-Glutamin oder GlutaMAX™. FluoroBrite™ DMEM wurde entwickelt, um das Signal-Rausch-Verhältnis der Fluorophore zu verbessern, so dass Forscher selbst die schwächsten Fluoreszenzvorgänge in einem Umfeld, das optimale Zellgesundheit fördert, erkennen können. Weitere Funktionen und Ausstattungsmerkmale:

• Verbesserung des Fluoreszenzsignals während der Lebendzell-Bildgebung
• DMEM-basiert zur Erhaltung der Zellgesundheit

Die Lebendzell-Fluoreszenzmikroskopie ist eine wesentliche Technik zur Visualisierung von fundamental wichtigen und physiologisch relevanten biologischen Ereignissen. Eine Herausforderung dieser Technik ist die Darstellungsfähigkeit schwacher Fluorophore, ohne Zellschädigungen, Photobleaching oder unerwünschte Nebenwirkungen auf die Zellviabilität zu bewirken. FluoroBrite™ DMEM ist für diese Fällen vorgesehen
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Konzentration1 X
Fertigungsqualität, HerstellungsqualitätcGMP-compliant under the ISO 13485 standard
ProdukttypDMEM (Dulbecco's Modified Eagle Medium)
Menge500 mL
Haltbarkeit12 Monate ab Herstellungsdatum
KlassifikationOhne Stoffe tierischen Ursprungs
FormFlüssig
Serum LevelStandard-Serumzugabe
SterilitätSteril gefiltert
Mit AdditivenHoher Glukosegehalt
Ohne AdditiveKein Glutamin, Keine HEPES, Kein Phenolrot, Ohne Natriumpyruvat
Unit SizeEach
Inhalt und Lagerung
Im Kühlschrank lagern (2 – 8 °C). Vor Licht schützen.

Häufig gestellte Fragen (FAQ)

What is the osmolality of Fluorobrite DMEM?

We do provide osmolality information on the certificate of analysis. All lots of Fluorobrite DMEM (Cat. Nos. A1896701 and A1896702) will meet the osmolality specification of 320-350 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (21)

Zitierungen und Referenzen
Abstract
Open source software for quantification of cell migration, protrusions, and fluorescence intensities.
Authors:Barry DJ, Durkin CH, Abella JV, Way M,
Journal:
PubMed ID:25847537
'Cell migration is frequently accompanied by changes in cell morphology (morphodynamics) on a range of spatial and temporal scales. Despite recent advances in imaging techniques, the application of unbiased computational image analysis methods for morphodynamic quantification is rare. For example, manual analysis using kymographs is still commonplace, often caused by ... More
A BRCA1-interacting lncRNA regulates homologous recombination.
Authors:Sharma V, Khurana S, Kubben N, Abdelmohsen K, Oberdoerffer P, Gorospe M, Misteli T,
Journal:
PubMed ID:26412854
Long non-coding RNAs (lncRNAs) are important players in diverse biological processes. Upon DNA damage, cells activate a complex signaling cascade referred to as the DNA damage response (DDR). Using a microarray screen, we identify here a novel lncRNA, DDSR1 (DNA damage-sensitive RNA1), which is induced upon DNA damage. DDSR1 induction ... More
Microtubule-dependent transport and dynamics of vimentin intermediate filaments.
Authors:Hookway C, Ding L, Davidson MW, Rappoport JZ, Danuser G, Gelfand VI,
Journal:
PubMed ID:25717187
We studied two aspects of vimentin intermediate filament dynamics-transport of filaments and subunit exchange. We observed transport of long filaments in the periphery of cells using live-cell structured illumination microscopy. We studied filament transport elsewhere in cells using a photoconvertible-vimentin probe and total internal reflection microscopy. We found that filaments ... More
Ebola Virus and Severe Acute Respiratory Syndrome Coronavirus Display Late Cell Entry Kinetics: Evidence that Transport to NPC1+ Endolysosomes Is a Rate-Defining Step.
Authors:Mingo RM, Simmons JA, Shoemaker CJ, Nelson EA, Schornberg KL, D'Souza RS, Casanova JE, White JM,
Journal:
PubMed ID:25552710
Ebola virus (EBOV) causes hemorrhagic fevers with high mortality rates. During cellular entry, the virus is internalized by macropinocytosis and trafficked through endosomes until fusion between the viral and an endosomal membrane is triggered, releasing the RNA genome into the cytoplasm. We found that while macropinocytotic uptake of filamentous EBOV ... More
3D imaging of Sox2 enhancer clusters in embryonic stem cells.
Authors:Liu Z, Legant WR, Chen BC, Li L, Grimm JB, Lavis LD, Betzig E, Tjian R,
Journal:
PubMed ID:25537195
Combinatorial cis-regulatory networks encoded in animal genomes represent the foundational gene expression mechanism for directing cell-fate commitment and maintenance of cell identity by transcription factors (TFs). However, the 3D spatial organization of cis-elements and how such sub-nuclear structures influence TF activity remain poorly understood. Here, we combine lattice light-sheet imaging, ... More
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