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FluoroBrite™ DMEM
Citations & References (21)
Gibco™

FluoroBrite™ DMEM

Gibco™ FluoroBrite™ DMEM features a background fluorescence that is comparable to PBS and 90% lower than that emitted by standard phenol red–free DMEM.
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Catalog NumberQuantity
A1896701500 mL
A189670210 x 500 mL
Catalog number A1896701
Price (USD)
61.65
Online Exclusive
65.75
Save 4.10 (6%)
Each
Quantity:
500 mL
Price (USD)
61.65
Online Exclusive
65.75
Save 4.10 (6%)
Each

Gibco™ FluoroBrite™ DMEM features a background fluorescence that is comparable to PBS and 90% lower than that emitted by standard phenol red–free DMEM. Formulated to include the required nutrients for routine cell culture when supplemented with 10% fetal bovine serum and 4 mM L-glutamine or GlutaMAX™ Supplement, FluoroBrite™ DMEM is designed to enhance the signal-to-noise ratio of fluorophores, enabling researchers to visualize even the weakest fluorescent events in an environment that promotes optimum cell health. Additional features include:

  • Enhancement of fluorescence signal during live-cell imaging
  • DMEM-based to help preserve cell health

Live-cell fluorescence microscopy is an essential technique for the visualization of fundamentally important and physiologically relevant biological events. A key challenge with this technique is the ability to image weak fluorophores without causing cell damage, photobleaching, or undesirable changes to cell health. FluoroBrite™ DMEM helps address these issues.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesHigh Glucose
Without AdditivesNo Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C). Protect from light.

Frequently asked questions (FAQs)

What is the osmolality of Fluorobrite DMEM?

We do provide osmolality information on the certificate of analysis. All lots of Fluorobrite DMEM (Cat. Nos. A1896701 and A1896702) will meet the osmolality specification of 320-350 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (21)

Citations & References
Abstract
Open source software for quantification of cell migration, protrusions, and fluorescence intensities.
Authors:Barry DJ, Durkin CH, Abella JV, Way M,
Journal:
PubMed ID:25847537
'Cell migration is frequently accompanied by changes in cell morphology (morphodynamics) on a range of spatial and temporal scales. Despite recent advances in imaging techniques, the application of unbiased computational image analysis methods for morphodynamic quantification is rare. For example, manual analysis using kymographs is still commonplace, often caused by ... More
A BRCA1-interacting lncRNA regulates homologous recombination.
Authors:Sharma V, Khurana S, Kubben N, Abdelmohsen K, Oberdoerffer P, Gorospe M, Misteli T,
Journal:
PubMed ID:26412854
Long non-coding RNAs (lncRNAs) are important players in diverse biological processes. Upon DNA damage, cells activate a complex signaling cascade referred to as the DNA damage response (DDR). Using a microarray screen, we identify here a novel lncRNA, DDSR1 (DNA damage-sensitive RNA1), which is induced upon DNA damage. DDSR1 induction ... More
Axonal autophagosomes recruit dynein for retrograde transport through fusion with late endosomes.
Authors:Cheng XT, Zhou B, Lin MY, Cai Q, Sheng ZH,
Journal:
PubMed ID:25940348
Efficient degradation of autophagic vacuoles (AVs) via lysosomes is an important cellular homeostatic process. This is particularly challenging for neurons because mature acidic lysosomes are relatively enriched in the soma. Although dynein-driven retrograde transport of AVs was suggested, a fundamental question remains how autophagosomes generated at distal axons acquire dynein ... More
The Nurr1 Activator 1,1-Bis(3'-Indolyl)-1-(p-Chlorophenyl)Methane Blocks Inflammatory Gene Expression in BV-2 Microglial Cells by Inhibiting Nuclear Factor ?B.
Authors:De Miranda BR, Popichak KA, Hammond SL, Jorgensen BA, Phillips AT, Safe S, Tjalkens RB,
Journal:
PubMed ID:25858541
NR4A family orphan nuclear receptors are an important class of transcription factors for development and homeostasis of dopaminergic neurons that also inhibit expression of inflammatory genes in glial cells. The identification of NR4A2 (Nurr1) as a suppressor of nuclear factor ?B (NF-?B)-related neuroinflammatory genes in microglia and astrocytes suggests that ... More
Posttranslational Modification of HOIP Blocks Toll-Like Receptor 4-Mediated Linear-Ubiquitin-Chain Formation.
Authors:Bowman J, Rodgers MA, Shi M, Amatya R, Hostager B, Iwai K, Gao SJ, Jung JU,
Journal:
PubMed ID:26578682
Linear ubiquitination is an atypical posttranslational modification catalyzed by the linear-ubiquitin-chain assembly complex (LUBAC), containing HOIP, HOIL-1L, and Sharpin. LUBAC facilitates NF-?B activation and inflammation upon receptor stimulation by ligating linear ubiquitin chains to critical signaling molecules. Indeed, linear-ubiquitination-dependent signaling is essential to prevent pyogenic bacterial infections that can lead ... More
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