Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555 - FAQs

View additional product information for Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555 - FAQs (A21127)

2 product FAQs found

What storage conditions would you recommend for secondary antibodies conjugated with fluorophores or HRP?

We recommend storing secondary antibodies conjugated with fluorophores or HRP at 4 degrees C as we have found that storage at -20 degrees C and the associated continuous freeze/thaw cycles can reduce the performance of fluorescent dyes. Storage at 4 degrees C improves fluorophore stability without affecting antibody performance.
You can also prepare small aliquots to avoid freeze/thaw cycles and store them at -20 degrees C, as with each freeze/thaw step the performance of the antibody would go down.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

I have two primary antibodies from the same host species that I wish to use to label the same cells. How do I choose secondary antibodies?

If the two primary antibodies are from different isotypes (such as mouse IgG1 and IgG2a), there are isotype-specific secondary antibodies to choose from. The other option is to make direct conjugates of the primary antibodies through the use of antibody label kits, reactive dyes, or custom conjugations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.