GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit
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GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit
The GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components neededRead more
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Catalog number A21175
Price (USD)/ 10 reactions
584.60
Special Offer
Online exclusive
Ends: 05-Nov-2024
790.00 
Save 205.40 (26%)
-
Add to cart
Price (USD)/ 10 reactions
584.60
Special Offer
Online exclusive
Ends: 05-Nov-2024
790.00 
Save 205.40 (26%)
Add to cart
GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit
Catalog numberA21175
Price (USD)/ 10 reactions
584.60
Special Offer
Online exclusive
Ends: 05-Nov-2024
790.00 
Save 205.40 (26%)
-
Add to cart
The GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads™ CD4 magnetic beads. Transfection efficiency can also be tracked using anti-CD4 fluorescent antibodies. The linearized GeneArt™ CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. A version with competent cells is also available (Cat. No. A21177).

The GeneArt™ CRISPR Nuclease Vector system offers:

• Easy–to-design genome engineering system
• Affordable, ready-to-use cloning vectors
• Enrichment for hard to transfect or difficult to modify cell lines

All-in-one Vector System for CRISPR-based Genome Editing
The GeneArt™ CRISPR Nuclease Vector kit offers a simple, ready-to-use, all-in-one expression vector system consisting of both a Cas9 nuclease expression cassette and a guide RNA (gRNA) cloning cassette for rapid and efficient cloning of DNA that encodes target-specific CRISPR RNA (crRNA). This system allows you to edit and engineer a genomic locus of choice in a sequence-specific manner from a single plasmid. After relevant targets have been identified with fast and easy-to-use GeneArt™ CRISPR vectors, the biologically relevant mutations can be precisely created with GeneArt™ Precision TALs, with high specificity and low off-target effects.

Need assistance with CRISPR gRNA design?
Our new CRISPR Search & Design tool allows you to search our database of >600,000 predesigned CRISPR gRNAs in human and mouse genes or analyze your sequence of interest for de novo gRNA designs using our proprietary algorithms. CRISPR sequences are optimized for gene knockout and target the first three transcribed exons for each gene. Search results include the top 6 CRISPR sequences with PAM sites, exon maps with gRNA binding sites, and potential off-target binding sites for each CRISPR sequence. The tool will design the correct gRNA format for your preferred CRISPR-Cas9 product, including oligos for your GeneArt™ CRISPR Nuclease Vector. Start designing today >
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cloning MethodRestriction Enzyme/MCS
Detection MethodPrimer-probe
FormLiquid
FormatKit
Reaction SpeedFast
TechniqueCRISPR-Cas9
Product LineGeneArt™
Product TypeCrispr Nuclease Vector With CD4 Enrichment Kit
PromoterU6, CMV
Protein TagCD4 fusion
Reporter GeneCD4
Sufficient For10 Reactions
For Use With (Application)Gene Expression, Cloning
No. of Reactions10 Reactions
Overhang3' overhangs
Starting MaterialOligos (DNA)
Unit Size10 reactions
Contents & Storage
Contains:
• CRISPR CD4 Nuclease Vector, linearized
• 10X Oligonucleotide Annealing Buffer
• DNase/RNase-free Water
• 5X Ligation Buffer
• T4 DNA Ligase
• U6 Forward Sequencing Primer
• Control Double-stranded Oligonucleotide

Store in freezer (-5 to -30°C).