Molecular Probes™ ATP Determination Kit offers a convenient bioluminescence assay for quantitative determination of ATP with recombinant firefly luciferase and its substrate D-luciferin. The assay is based on luciferase’s absolute requirement for ATP in producing light (emission maximum ∼560 nm at pH 7.8). This assay is extremely sensitive; most luminometers can detect as little as 0.1 picomole of pre-existing ATP, or ATP as it is being formed in kinetic systems. This sensitivity has led to numerous applications for detecting ATP production in various enzymatic reactions, including ATPase and NADPH oxidase, as well as for detecting low-level bacterial contamination in samples such as blood, milk, urine, soil and sludge. The luciferin–luciferase bioluminescence assay has also been used successfully to study the effects of antibiotics on bacterial populations, to determine cell proliferation and cytotoxicity in both bacterial and mammalian cells, and to distinguish cytostatic versus cytocidal potential of anticancer drugs on malignant cell growth.

ATP Determination Kit Specifications:
• Luminescence is monitored at ∼560 nm using a luminometer
• Can detect as little as 0.1 picomole of pre-existing ATP, or ATP as it is being formed in kinetic systems
• Luciferase and luciferin are packaged separately, which enables researchers to optimize conditions for their samples and instruments
• Includes sufficient reagents to perform 200 ATP assays using 500 µL sample volumes or 1,000 ATP assays using 100 µL sample volumes

Find More Viability and Cytotoxicity Assays and Probes for Detecting Enzymes That Metabolize Phosphates
Review Viability and Cytotoxicity Assay Kits for Diverse Cell Types—Section 15.3 and Detecting Enzymes That Metabolize Phosphates and Polyphosphates—Section 10.3 in the Molecular Probes™ Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.