Amplex® Red Uric Acid/Uricase Assay Kit

Catalog number: A22181

Molecular Probes™  Related applications: Protein Assays and Analysis

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Description

The Amplex® Red Uric Acid/Uricase Assay Kit provides a sensitive and simple method for detecting uric acid or uricase using either a fluorescence microplate reader or fluorometer.

See our complete line of Fluorescence Microplate assays.

• Detect concentrations as low as 100 nM uric acid or 0.2 mU/mL of uricase activity
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference

Serum uric acid is the end product of purine metabolism in the body tissues and is cleared through the kidneys by glomerular filtration. Most animals can metabolize uric acid to more readily excreted products, but humans lack the necessary enzyme, urate oxidase (uricase), as a result of the presence of two “nonsense mutations” in the human gene for uricase.

The Amplex® Red Uric Acid/Uricase Assay Kit provides an ultrasensitive method for detecting uric acid or for monitoring uricase activity. In the assay, uricase catalyzes the conversion of uric acid to allantoin, hydrogen peroxide, and carbon dioxide. In the presence of horseradish peroxidase (HRP), hydrogen peroxide reacts stoichiometrically with the Amplex® Red reagent to generate the red-fluorescent oxidation product resorufin.

Because resorufin has absorption and fluorescence emission maxima of approximately 571 nm and 585 nm, respectively, there is little interference from autofluorescence in most biological samples.

Use Amplex® Red Assays for a Broad Range of Investigations
A wide variety of validated Amplex® Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex® UltraRed Reagent (Cat. No. A36006), a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex® Red/UltraRed Stop Reagent (Cat. No. A33855). The Amplex® Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours. Custom assay design and packaging are also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Microplate Reader, Spectrophotometer, Fluorometer
Detection Method: Fluorescent
Format: Tube(s), 96-well plate
Label or Dye: Amplex® Red
Product Size: 400 assays
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

Documents

Manuals & protocols