Foxp3 Transcription Factor Wash Buffer

Foxp3 Transcription Factor Wash Buffer - FAQs

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5 product FAQs found

What kind of BSA should I use for diluting the 10X stock solution of Foxp3 Transcription Factor Wash Buffer to a 1X working concentration?

The BSA should be Fraction V, defatted BSA. 'Fraction V' is derived from Cohn method of fractionation using cold ethanol precipitation. The use of chilled ethanol in the Cohn fractionation process preserves the native albumin structure.

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The manual recommends diluting the 10X stock solution of Foxp3 Transcription Factor Wash Buffer to a 1X working concentration in PBS containing 1% BSA. Can I use other species of serum albumin in lieu of bovine serum albumin?

We have not tested this with serum albumins from other species, but it may work. The serum albumin should be highly purified, delipidated (Fraction V defatted), and free of antibodies.

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What are the components/formulation of the Foxp3 Transcription Factor Wash Buffer?

The identity of the components of the Foxp3 Transcription Factor Wash Buffer and the formulation are proprietary.

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The Foxp3 Transcription Factor Wash Buffer is specially formulated to work with the APC, FITC, and PE conjugates of Foxp3 Mouse Anti-Mouse Monoclonal Antibody (clone 3G3). Can I use it with other Foxp3 antibodies labeled with different fluorophores?

Yes, the Foxp3 Transcription Factor Wash Buffer can be used with other Foxp3 antibodies labeled with fluorophores besides APC, FITC, and PE

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The Foxp3 Transcription Factor Wash Buffer is specially formulated to work with the APC, FITC, and PE conjugates of Foxp3 Mouse Anti-Mouse Monoclonal Antibody (clone 3G3). Can I use it for other intracellular or intranuclear markers?

Yes. Although we have not tested the Foxp3 Transcription Factor Wash Buffer with other antibodies for intracellular/intranuclear labeling for flow cytometry, it should work.

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