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Human Neural Stem Cell Immunocytochemistry Kit - FAQs

Human Neural Stem Cell Immunocytochemistry Kit - FAQs

View additional product information for Human Neural Stem Cell Immunocytochemistry Kit - FAQs (A24354)

4 product FAQs found

Can I purchase the primary antibodies from the Human Neural Stem Cell Immunocytochemistry Kit (Cat. No. A24354) separately?

Yes, the primary antibodies from the Human Neural Stem Cell Immunocytochemistry Kit (Cat. No. A24354) can be purchased separately. Here are the catalog numbers:
  • Nestin Monoclonal Antibody (10C2), eBioscience (Cat. No. 14984382)
  • SOX2 Polyclonal Antibody (Cat. No. 481400)
  • PAX6 Polyclonal Antibody (Cat. No. 426600)
  • SOX1 Goat anti-Human, Mouse, Rat, Polyclonal, R&D Systems; available through Fisher Scientific (Cat. No. AF3369)

The concentrations of the antibodies in the Human Neural Stem Cell Immunocytochemistry Kit are proprietary. If you purchase the above antibodies separately, you will need to determine the best concentrations to use in your assay.

Find additional tips, troubleshooting help, and resources within our Cell Imaging Support Center

The Human Neural Stem Cell Immunocytochemistry Kit provides the NucBlue Fixed Cell Stain. If I don't have the correct filter set for this stain, can I use another nucleic acid stain?

If you use Alexa Fluor 488/Alexa Fluor 555 dye-conjugated secondary antibodies and you have a Cy5/Alexa Fluor 647 filter set, you may use a far-red nucleic acid stain, such as NucRed Dead 647 ReadyProbes Reagent (Cat. No. R37113) or SYTOX Deep Red Nucleic Acid Stain (Cat. No. S11380). Do not use these far-red nucleic acid stains if using an Alexa Fluor 594 dye-conjugated secondary antibody as there is too much spectral overlap of Alexa Fluor 594 into the Cy5/Alexa Fluor 647 range.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the Human Neural Stem Cell Immunocytochemistry Kit for flow cytometry analysis?

We do not recommend using the Human Neural Stem Cell Immunocytochemistry Kit for flow cytometry analysis. The kit and protocol were specifically developed for imaging analysis (microscopy/high-content screening (HCS)).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer the individual components of the Human Neural Stem Cell Immunocytochemistry Kit as standalone products?

Only the NucBlue Fixed Cell ReadyProbes Reagent may be purchased separately as Cat. No. R37606. We do offer the secondary antibodies separately, but not in the same proprietary concentration as provided in the kit. We also offer formaldehyde, DPBS, BSA, and saponin as separate catalog numbers, but not in the volumes or as formulated in the kit. The primary antibodies are not available as separate stock items; all non-stock items in the kit may be available as a custom order. Please contact your Account Representative.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.