Mouse (ICR) Inactivated Embryonic Fibroblasts

      Mouse (ICR) Inactivated Embryonic Fibroblasts

      Numéro de catalogue:  A24903

      Gibco™  Applications liées: Stem Cell Culture

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      Description

      Mouse embryonic fibroblasts (MEFs) are used as a feeder layer when culturing mouse and human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). They are isolated from mouse (strain ICR) embryos. The cells are expanded and inactivated by γ irradiation and cryopreserved. Prior to cryopreservation, the cells are tested for viability (>80% post-thaw viability), verified for growth arrest by plating inactivated cells at low density and monitoring for foci of cell growth after 14 days in culture, and also tested for their ability to support growth of mouse embryonic stem cells (mESCs), human embryonic stem cells (hESCs), and marker expression (Oct 4).
      For Research Use Only. Not for use in diagnostic procedures.

      Spécifications

      Age: Fetal
      Cell Type: Feeder Cells
      Donor Source: Pooled
      Form: Frozen
      Product Size: 1 mL
      Species: Mouse

      Contenu et stockage

      Store in liquid nitrogen.

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