: The Ion PGM™ Hi-Q™ Sequencing Kit will be discontinued in Q1 of 2017. The supported replacement kit, the Ion PGM™ Hi-Q™ View Sequencing Kit (A30044), is now available for order. The final build of the Ion PGM™ Hi-Q™ Sequencing Kit will be Q1 of 2017 and will have a 7–8 month shelf life when stocked. Based on in-stock availability, this will allow for migration to the Ion PGM™ Hi-Q™ View Sequencing Kit (A30044) until the end of 2017.

Ion PGM™ Hi-Q™ Sequencing Kit

Catalog number: A25592

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


The Ion PGM™ Hi-Q™ Sequencing Kit contains reagents and consumables for robust and highly accurate sequencing of 200-and 400-base pair libraries using the Ion OneTouch™ 2 System combined with the Ion PGM™ System. This kit offers the most advanced sequencing chemistry available to users of the Ion PGM™ System, and its economical price brings next-generation sequencing capability to every lab. Use the Ion PGM™ Hi-Q™ Sequencing Kit to maximize your variant calling performance.

Note: an Ion PGM™ Wash 2 Bottle Kit is required for use of this sequencing kit. One Wash 2 Bottle kit may be used for up to 40 sequencing runs before replacement.

• Delivers the latest advancements to data quality and accuracy using the Ion PGM™ System
• Provides a single unified solution for both 200- and 400-base pair sequencing
• Offers low cost per sample through the use of multiplexing

Maximize your results with high accuracy sequencing chemistry
The Ion PGM™ Hi-Q™ Sequencing Kit is configured to support all of your sequencing needs for applications requiring 200 bp or 400 bp read lengths. It is for use with the Ion 314™ Chip v2, Ion 316™ Chip v2, or Ion 318™ Chip v2 and is compatible with the Ion PGM™ Template OT2 200 and Ion PGM™ Template OT2 400 kits.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Ion PGM™ System
Recommended Applications: Comparative Sequencing (Germline Mutations - 50:50), Comparative Sequencing (Somatic Mutations - 10:50), De Novo Sequencing, Comparative Sequencing (Germline Mutations - 10:90 Heterozygotes)
Product Size: 1 kit
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice

Contents & storage

Contains reagents for twelve 100 bp, eight 200 bp, or four 400 bp sequencing reactions using the Ion PGM™ System.

Components include sequencing supplies, enzymes, nucleotides, spike-in sequencing controls, and sequencing reagents.

Store components at -20°C, 4°C, or room temperature, as indicated.


Manuals & protocols

Recommended products