: The Ion PGM™ Hi-Q™ Sequencing Kit will be discontinued in Q1 of 2017. The supported replacement kit, the Ion PGM™ Hi-Q™ View Sequencing Kit (A30044), is now available for order. The final build of the Ion PGM™ Hi-Q™ Sequencing Kit will be Q1 of 2017 and will have a 7–8 month shelf life when stocked. Based on in-stock availability, this will allow for migration to the Ion PGM™ Hi-Q™ View Sequencing Kit (A30044) until the end of 2017.

Ion PGM™ Hi-Q™ Sequencing Kit

Catalog number: A25592

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

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The Ion PGM™ Hi-Q™ Sequencing Kit contains reagents and consumables for robust and highly accurate sequencing of 200-and 400-base pair libraries using the Ion OneTouch™ 2 System combined with the Ion PGM™ System. This kit offers the most advanced sequencing chemistry available to users of the Ion PGM™ System, and its economical price brings next-generation sequencing capability to every lab. Use the Ion PGM™ Hi-Q™ Sequencing Kit to maximize your variant calling performance.

Note: an Ion PGM™ Wash 2 Bottle Kit is required for use of this sequencing kit. One Wash 2 Bottle kit may be used for up to 40 sequencing runs before replacement.

• Delivers the latest advancements to data quality and accuracy using the Ion PGM™ System
• Provides a single unified solution for both 200- and 400-base pair sequencing
• Offers low cost per sample through the use of multiplexing

Maximize your results with high accuracy sequencing chemistry
The Ion PGM™ Hi-Q™ Sequencing Kit is configured to support all of your sequencing needs for applications requiring 200 bp or 400 bp read lengths. It is for use with the Ion 314™ Chip v2, Ion 316™ Chip v2, or Ion 318™ Chip v2 and is compatible with the Ion PGM™ Template OT2 200 and Ion PGM™ Template OT2 400 kits.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Ion PGM™ System
Recommended Applications: Comparative Sequencing (Germline Mutations - 10:90 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), Comparative Sequencing (Somatic Mutations - 10:50), De Novo Sequencing
Product Line: Ion PGM™
Product Size: 1 kit
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice

Contents & storage

Contains reagents for twelve 100 bp, eight 200 bp, or four 400 bp sequencing reactions using the Ion PGM™ System.

Components include sequencing supplies, enzymes, nucleotides, spike-in sequencing controls, and sequencing reagents.

Store components at -20°C, 4°C, or room temperature, as indicated.


Manuals & protocols

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