Search

  • Contact Us
  • Quick Order
Fetal Bovine Serum, exosome-depleted - FAQs

Fetal Bovine Serum, exosome-depleted - FAQs

View additional product information for Fetal Bovine Serum, exosome-depleted - FAQs (A25904DG)

6 product FAQs found

What cells can be cultured in Exosome-Depleted FBS, and at what concentration?

We have specifically tested Jurkat, MCF-7, HeLa, PC3, HEK293, and A549 cells, all of which exhibited acceptable performance. HeLa cells showed the most sensitivity to the presence of Exosome-depleted FBS. Adjustments may be needed for the concentration of Exosome-depleted FBS to match the requirements of the specific cell line and system. We recommend starting at 10% Exosome-depleted FBS, and adjusting up or down from there as necessary. Also, the Exosome-depleted FBS has been tested and validated for a single passage of cells for 48 hours (and we have also tested HeLa, MCF-7, and HEK293 cells successfully up to 96 hours of culture).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How is Exosome-depleted FBS manufactured?

We have developed a proprietary process to remove exosomes and maintain as much of the growth performance as possible.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw and refreeze Exosome-depleted FBS?

Yes, this product has been tested to ensure there is no loss of product performance after thawing, aliquoting, and refreezing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What does "depleted" in Exosome-depleted FBS mean?

The Exosome-depleted FBS that we ship to you will contain ≤10% of the exosomes present in the starting material FBS. We use an in-house fluorescence assay specifically developed for exosome quantification to confirm this level of depletion in every lot. See the scientific poster at thermofisher.com/exosomedepletedfbs

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there anything different about the quality of Exosome-depleted FBS compared to other FBS products?

Exosome-depleted FBS undergoes full sterility testing like our other sera products. It is subjected to additional tests for exosome depletion and a specific cell culture performance test.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

When would I use Exosome-depleted FBS?

We recommend using this product for exosome-specific assays, but you may also use it to culture the cells prior to the assay to minimize background signaling in your cells. We recommend determining the concentration to use, but 5-10% FBS is the most common.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.