: The Ion PGM™ Hi-Q™ Chef Kit will be discontinued in Q1 of 2017. The supported replacement kit, the Ion PGM™ Hi-Q™ View Chef Kit (A29902), is now available for order. The final build of the Ion PGM™ Hi-Q™ Chef Kit will be Q1 of 2017 and will have a 7–8 month shelf life when stocked. Based on in-stock availability, this will allow for migration to the Ion PGM™ Hi-Q™ View Chef Kit (A29902) until the end of 2017.
Notice: The Ion PGM™ Calibration Standard (Cat. No. A27832) is no longer included in the Ion PGM Hi-Q Chef Kit. If you are performing de novo sequencing experiments that do not include a reference BAM file, please separately order the Ion PGM™ Calibration Standard (Cat. No. A27832).

Ion PGM™ Hi-Q™ Chef Kit

Catalog number: A25948

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

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The Ion PGM™ Hi-Q™ Chef Kit enables accurate and reproducible template preparation, chip loading, and sequencing of 400 bp (with additional purchase*) and 200 bp libraries on the Ion Chef™ System and is compatible with the Ion PGM™ System. The plug & play, pre-packaged, single-use reagent cartridges enable a fully automated workflow, processing two samples per run for increased productivity. Reagent and sample barcoding features help to verify the user-defined run plan and offer complete run traceability.

• Delivers automated template preparation and chip loading for 400 and 200 base pair libraries
• Provides the most accurate sequencing chemistry available for the Ion PGM™ System
• Designed to minimize the hands on time required to prepare your samples
• Helps increase lab throughput with dual sample processing

Maximize your results with high accuracy sequencing chemistry
The Ion PGM™ Hi-Q™ Chef Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow. Designed to simplify the overall workflow with minimal hands-on time, the Ion Chef™ System is ideal for users of all experience levels. The Ion PGM™ Hi-Q™ Chef Kit contains the most advanced templating and sequencing chemistries available to Ion PGM™ customers. Use the Ion PGM™ Hi-Q™ Sequencing Kit to help maximize your variant calling performance and improve de novo sequencing applications.

A single kit to meet your Ion PGM™ sequencing needs
The Ion PGM™ Hi-Q™ Chef Kit is configured to support all of your sequencing needs for applications requiring 200 bp or 400 bp* read lengths. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, and the Ion 318™ Chip v2 BC. The kit includes sufficient reagents for eight 200 bp sequencing reactions.

*For 400-base applications, order the Ion PGM™ Hi-Q™ Chef Supplies 400 Kit, which provides the required additional reagents needed for eight 400 bp sequencing reactions.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Ion Chef™ System, Ion PGM™ System
Recommended Applications: Standard Sequencing
Product Line: Ion Torrent™
Product Size: 8 reactions

Contents & storage

Components include template reagents, enzyme mixes, nucleotides, spike-in sequencing controls, solutions & buffers, consumables, and supplies.


Manuals & protocols

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