Thermo Scientific™

Oncomine™ Solid Tumour DNA Kit

Référence: A26761
Thermo Scientific™

Oncomine™ Solid Tumour DNA Kit

Référence: A26761
Référence
A26761
Taille de l’unité
96 reactions
RéférenceTaille de l’unitéPrix (EUR)
A2676196 reactionsDemander un devis
Présentation du produit
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The Oncomine™ Solid Tumour DNA Kit consists of a single pool of primers and associated reagents to perform multiplex PCR for preparation of amplicon libraries for next-generation sequencing (NGS) and is designed to provide sensitive and comprehensive sample amplification. Sufficient reagents are supplied for 96 reactions, together with bar codes facilitating the combination of up to 16 samples in a single NGS run.

Intended use
The Oncomine™ Solid Tumour DNA Kit provides material that captures regions of human somatic variants (deletions, insertions, inversions, and substitutions) present in selected regions in the following cancer related genes; EGFR, ALK, ERBB2, ERBB4, FGFR1, FGFR2, FGFR3, MET, DDR2, KRAS, PIK3CA, BRAF, AKT1, PTEN, NRAS, MAP2K1, STK11, NOTCH1, CTNNB1, SMAD4, FBXW7, TP53 for analysis using next-generation sequencing technology. The Solid Tumour DNA kit is intended for in vitro diagnostic use by trained personnel in a professional laboratory environment with extracted human DNA samples including those from formalin-fixed paraffin-embedded (FFPE) tissues.

The Oncomine™ Solid Tumour DNA Kit amplifies genomic 'hot spot' regions that are frequently mutated in human cancer genes. The diagnostic kit offers:
• Compatibility with FFPE samples with as little as 10 ng of input DNA
• Uniform coverage for more efficient sequencing and helps save costs
• Primer sets optimized through enhanced design aiming to provide highly specific and uniform target enrichment
• A simple workflow to produce targeted libraries in typically 3.5 hours

The Oncomine™ Solid Tumour DNA Kit is designed to amplify 92 amplicons covering more than 1800 COSMIC mutations from 22 genes associated with solid tumours. The optimized and validated primer designs enable coverage uniformity, so a minimum required depth across all amplicons can be achieved using minmal sequencing throughput. Scalability and flexibility are facilitated through the ability to sequence one sample or multiplex up to 16 barcoded samples during amplification.

The Oncomine™ Solid Tumour DNA Kit can be used together with the Oncomine™ Solid Tumour Fusion Transcript Kit on the same FFPE sample, with the two individual library preparations being combined for NGS.

This kit was developed in association with The OncoNetwork Consortium.

Spécifications

Format
Kit
Type d’échantillon
FFPE
Sequencing Type
Genome and DNA Sequencing
Type de produit final
Genomic DNA (Targeted Regions)
À utiliser avec (application)
Sequencing
Bibliothèques
Targeted Sequencing Library
Nbre de réactions
96
Gamme de produits
Oncomine™
Type de produit
Solid Tumour DNA Kit
Conditions d’expédition
Approved for shipment on Wet or Dry Ice
Espèces
Human
Étape du flux de travail
Library Generation

Contenu et stockage

Library box: store at -5 to -30°C.
Equalizer box: store at 2–8°C.

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