Ion AmpliSeq™ Exome RDY Kit 1x8

Catalog number: A27192

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

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Description

The Ion AmpliSeq™ Exome RDY Kit enables the fastest and simplest method of exome enrichment, now even easier with oligo pools for ultra-high multiplex PCR exome enrichment dried down in a 96-well plate. With half the number of target amplification pipetting steps compared to the original protocol and the elimination of small pipetting volumes, exome libraries can now be produced with unparalleled ease in under 6 hours with less than 50 minutes of hands-on time. Also, the Ion AmpliSeq™ Exome RDY Kit is compatible with the Ion Library Equalizer™ Kit for ease in library normalization. Leveraging the ultra-high multiplex PCR approach of Ion AmpliSeq™ technology with template preparation on the Ion Chef™ System along with the Ion Proton™ Sequencer, the Ion AmpliSeq™ Exome RDY Kit allows for rapid sequencing of key exonic regions of the genome, going from DNA to variants in just 2 days.

The Ion AmpliSeq™ Exome RDY Kit 1x8 configuration includes the following components for 8 exomes (running 2 exomes per Ion PI™ –Chip v3):
• Ion AmpliSeq™ Exome RDY Panel: dried down oligo pools/primers in one 96-well plate with all 8 rows filled (1x8), for ultra-high multiplex PCR enrichment of the exonic regions of the genome
• Ion AmpliSeq™ Library Kit Plus (8 exomes) for robust library preparation of Ion AmpliSeq™ Exome libraries using Ion AmpliSeq™ technology
• Ion PI™ Chip Kit v3 for sequencing on the Ion Proton™ Sequencer

The Ion AmpliSeq™ Exome RDY Kit enables you to:
• Simplify exome enrichment through the combination of dried-down primers and the Ion AmpliSeq™ workflow, resulting in less than 1 hour of hands-on time to produce exome libraries in under 6 hours
• Accelerate your disease research with rapid exome sequencing, going from DNA to variants in just 2 days
• Access affordable exome sequencing when you need it, without the need to batch dozens of samples or the high price of a lower-throughput run
• Maximize sequencing efficiency with >90% on-target bases and >92% coverage uniformity, and design covering >97% of the Consensus Coding Sequences (CCDS) annotation
• Identify annotated, filtered variants without bioinformatics expertise through fully integrated analysis with Torrent Suite™ and Ion Reporter™ software

Quickest exome enrichment with unprecedented simplicity
The Ion AmpliSeq™ Exome RDY Kit 1x8 configuration provides unmatched simplicity for exome enrichment. The same primers used in the Ion AmpliSeq™ Exome Kit 4xDuo and Ion AmpliSeq™ Exome Kit 8xTrio have been dried down into one 96-well plate, using the 12 wells of a single row for each of the 12 primer pools, and with 8 rows in total, leading to the use of one row per exome. The kit is sufficient to prepare 8 exome libraries. Additionally, the dried down primers contain a light blue dye for easy identification of used or occupied rows. The number of target amplification pipetting steps is reduced by 46%, and small pipetting volumes (<5 µL) have been eliminated. With a workflow requiring less than 50 minutes of hands-on time and no centrifugation steps, the kit enables exome library preparation in less than 6 hours with the least amount of hands-on time. Additionally, the Ion AmpliSeq™ Exome RDY Kit maintains a low input requirement of 50 ng DNA. With the included Ion AmpliSeq™ Library Kit Plus, exome library preparation is more robust, resulting in higher uniformity and consistently higher library yield when performing library amplification. Also, the Ion AmpliSeq™ Exome RDY Kit is now compatible with the Ion Library Equalizer™ Kit for ultimate ease in library normalization.

Lowest cost per exome using a benchtop sequencer
Paired with the Ion Proton™ System, the Ion AmpliSeq™ Exome RDY Kit allows disease researchers to access affordable exome sequencing on their benchtops without long turn-around times or the sample batching normally required to achieve a low cost per sample. Using the Ion AmpliSeq™ Exome RDY Kit with the Ion Proton™ System enables you to go from DNA to variants in as little as 2 days. Additionally, with the most uniform exome enrichment covering >97% of the CCDS, you can achieve the most efficient sequencing with 2 exomes run per Ion PI™ chip.

Fully integrated analysis without bioinformatics expertise
Use of the kit with Torrent Suite™ and Ion Reporter™ software provides a simple and streamlined analysis workflow that automates the identification of your variants of interest without needing the expertise or infrastructure of a full bioinformatics team. Additionally, the Ion Proton™ Sequencer along with Ion Reporter™ Software deliver both SNP and indel information along with copy number changes through a single analysis workflow, advancing your human disease research.

Note:
This kit is compatible with the Ion Chef™, Ion OneTouch™ 2, and Ion Proton™ systems
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Ion Proton™ System
Library Type: Targeted Sequencing Library
Workflow Step: Library Barcoding, Library Generation, PCR Amplification
Starting Material: DNA
Sequencing Type: Genome & DNA Sequencing
Product Size: 8 reactions

Contents & storage

• Ion AmpliSeq Exome RDY Panel
• Ion PI Chip Kit V3 4-pack
Store the above at room temperature.

• Ion AmpliSeq Library Kit Plus
Store the above at -5 to -30°C.

Documents

Manuals & protocols