The Ion PI™ Hi-Q™ Chef Kit enables robust and reproducible template preparation, chip loading, and sequencing of 200 base-read libraries on the Ion Chef™ System as part of the Ion Proton™ System sequencing workflow. The plug & play, pre-packaged, single-use reagent cartridges for template and chip preparation enables an automated workflow for processing single and dual samples. The reagent and sample barcodes interact with the Ion Chef™ System to validate user-defined run plans and offer complete run traceability.
The Ion PI™ Hi-Q™ Chef kit offers: • The latest advancements in data quality and accuracy using the Ion Proton™ System • Improved specificity for SNP and insertion/deletion variant calls • Increased sensitivity with improved target coverage and representation • A simplified workflow with ready-to-use Ion PI™ chips v3 • Over 60% reduction of library input amount when compared to PI™ IC 200 workflow • Less than 15 minutes hands-on time • Protocols for the preparation of one or two Ion PI™ chips per run • Planned run sharing between different Ion Chef™ systems
The Ion PI™ Hi-Q™ Chef Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow, designed to simplify and streamline the overall workflow with minimal hands-on-time for users with any experience level. This easy-to-use kit delivers high-quality and reproducible templated products for semiconductor sequencing.
The Ion PI™ Hi-Q™ Chef Kit contains sufficient reagents and consumables for 8 template preparation and sequencing runs, and should be used in conjunction with Ion PI™ chips v3 for sample tracking purposes.
Notes: • This kit supports 200 base-read libraries for a variety of applications. • This kit is only compatible with the Ion Chef™ and Ion Proton™ systems. • Components of this kit have been optimized for use on the Ion Chef™ and Ion Proton™ systems specifically and should NOT be replaced or interchanged with other product kit components.