New Next-Generation Sequencing Support Center—find technical tips and troubleshooting recommendations from our scientists.
Ion Torrent™

Ion PI™ Hi-Q™ Chef Kit

Catalog number:  A27198

Related applications:


Back to top


The Ion PI™ Hi-Q™ Chef Kit enables robust and reproducible template preparation, chip loading, and sequencing of 200 base-read libraries on the Ion Chef™ System as part of the Ion Proton™ System sequencing workflow. The plug & play, pre-packaged, single-use reagent cartridges for template and chip preparation enables an automated workflow for processing single and dual samples. The reagent and sample barcodes interact with the Ion Chef™ System to validate user-defined run plans and offer complete run traceability.

The Ion PI™ Hi-Q™ Chef kit offers:
• The latest advancements in data quality and accuracy using the Ion Proton™ System
• Improved specificity for SNP and insertion/deletion variant calls
• Increased sensitivity with improved target coverage and representation
• A simplified workflow with ready-to-use Ion PI™ chips v3
• Over 60% reduction of library input amount when compared to PI™ IC 200 workflow
• Less than 15 minutes hands-on time
• Protocols for the preparation of one or two Ion PI™ chips per run
• Planned run sharing between different Ion Chef™ systems

The Ion PI™ Hi-Q™ Chef Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow, designed to simplify and streamline the overall workflow with minimal hands-on-time for users with any experience level. This easy-to-use kit delivers high-quality and reproducible templated products for semiconductor sequencing.

The Ion PI™ Hi-Q™ Chef Kit contains sufficient reagents and consumables for 8 template preparation and sequencing runs, and should be used in conjunction with Ion PI™ chips v3 for sample tracking purposes.

• This kit supports 200 base-read libraries for a variety of applications.
• This kit is only compatible with the Ion Chef™ and Ion Proton™ systems.
• Components of this kit have been optimized for use on the Ion Chef™ and Ion Proton™ systems specifically and should NOT be replaced or interchanged with other product kit components.
WARNING: Reproductive Harm -
For Research Use Only. Not for use in diagnostic procedures.


Recommended Applications: Resequencing
For Use With (Equipment): Ion Proton™ System

Contents & storage

• Ion PI Hi-Q Chef Supplies
• Ion PI Hi-Q Chef Solutions
• Ion PI Seq Supplies 200
Store the above at room temperature.

• Ion PI Hi-Q Chef Reagents
• Ion PI Sequencing Nucleotides
Store the above at -5 to -30°C.

• Ion PI Hi-Q Seq 200 Solutions
Store the above at 2–8°C.