Fetal Bovine Serum, exosome-depleted

Name: Fetal Bovine Serum, exosome-depleted
SKU: A2720803

Gibco™ Exosome-Depleted FBS streamLines and standardizes cell culture workflows for exosome research. It is ready to use and performance tested to help save time and enable optimal exosome preparations.

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製品番号（カタログ番号）	数量	フォーマット
A2720803	50 mL	One Shot
A2720801	500 mL	Bottle

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製品番号（カタログ番号） A2720803

価格（JPY）

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数量:

50 mL

フォーマット:

One Shot

Lowest exosome background
Enables consistent results: each lot tested for cell culture performance
No preparation needed: ultracentrifugation is not required

Exosome-Depleted FBS has been depleted of ≥90% of endogenous exosomes and is intended for use in cell culture exosome isolation assays. Every lot is tested for exosome depletion and culture performance, and our standard quality control assays are conducted to help ensure optimal and consistent results. This ready-to-use FBS for cell culture exosome isolations helps eliminate the risks associated with FBS reagent preparation by ultracentrifugation, for optimal assay results and increased experimental throughput.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.

仕様

製品ラインOne Shot

出荷条件凍結

種Bovine

Age胎児

原産国米国

形状Liquid

フォーマットOne Shot

製品タイプFetal Bovine Serum

数量50 mL

Serum TreatmentExosome-depleted

無菌性Sterile

Unit SizeEach

組成および保存条件

保存条件：≤-10°C
輸送条件: 凍結

よくあるご質問（FAQ）

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Have you tested Exosome-Depleted FBS for mycoplasmas?

Yes, the material undergoes USP sterility testing, which includes mycoplasma testing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the source material for Exosome-Depleted FBS?

Exosome-Depleted FBS is made from Gibco FBS (qualified, US-origin) in the Value category.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can serum be stored at 2-8 degrees C?

Serum can be kept at 2-8 degrees C for up to 4 weeks.

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Are there any other packaging configurations available for the new One Shot FBS product?

Yes, we also offer a 10 X 50 mL package, which is equivalent to a 500 mL bottle of FBS.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, exosome-depleted FAQs