Notice: The Ion PGM™ Calibration Standard (Cat. No. A27832) is no longer included in the Ion PGM Hi-Q OT2 Kit. If you are performing de novo sequencing experiments that do not include a reference BAM file, please separately order the Ion PGM™ Calibration Standard (Cat. No. A27832).

Notice
: The Ion PGM™ Hi-Q™ OT2 kit will be discontinued in Q1 of 2017. The supported replacement kit, the Ion PGM™ Hi-Q™ View OT2 Kit (A29900), is now available for order. The final build of the Ion PGM™ Hi-Q™ OT2 kit will be Q1 of 2017 and will have a 7–8 month shelf life when stocked. Based on in-stock availability, this will allow for migration to the Ion PGM™ Hi-Q™ View OT2 Kit (A29900) until the end of 2017.
 

Ion PGM™ Hi-Q™ OT2 Kit

Catalog number: A27739

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

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Description

The Ion PGM™ Hi-Q™ OT2 Kit enables accurate and reproducible template preparation for libraries up to 400 base pairs using the Ion OneTouch™ 2 System and is compatible with the Ion PGM™ System. As an integral component of the semiconductor sequencing workflow, the Ion PGM™ Hi-Q™ OT2 Kit is designed to produce high-quality Ion Sphere™ particles and is designed for use in combination with the Ion PGM™ Hi-Q™ Sequencing Kit.

• Delivers template preparation for 400 and 200 base pair libraries
• Designed to provide the most accurate templating chemistry available for the Ion PGM™ System
• Offers templating controls to help ensure high quality sequencing results
• Enables increased lab throughput with multiplexing support

Maximize your results with high accuracy templating chemistry
The Ion PGM™ Hi-Q™ OT2 Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow. Designed for users of the Ion OneTouch™ 2 system, the Ion PGM™ Hi-Q™ OT2 Kit is optimized for users looking to maximize their sequencing data quality. The Ion PGM™ Hi-Q™ OT2 Kit contains the most advanced templating chemistry available to Ion PGM™ customers. Use the Ion PGM™ Hi-Q™ OT2 Kit to maximize your variant calling performance and improve de novo sequencing research applications.

A single kit to meet your Ion PGM™ sequencing needs
The Ion PGM™ Hi-Q™ OT2 Kit is configured to support your sequencing needs for applications requiring 200 bp or 400 bp read lengths. When combined with the Ion PGM™ Hi-Q™ Sequencing Kit, the Ion PGM™ Hi-Q™ OT2 Kit offers optimal performance. This kit supports the Ion PGM™ 3-series chips, including: Ion 314™ Chip v2 BC, Ion 314™ Chip v2, Ion 316™ Chip v2 BC, Ion 316™ Chip v2, Ion 318™ Chip v2 BC, and Ion 318™ Chip v2. The kit includes sufficient reagents for eight templating reactions. The kit components include Ion Sphere™ particles, buffers, reagents, and supplies.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: Ion Torrent™
Product Size: 8 reactions

Contents & storage

The kit includes Ion Sphere™ particles, buffers, reagents, and supplies.

Documents

Manuals & protocols