: The Ion ReproSeq™ 316 without Chips will be discontinued in Q1 of 2017. The final build of the Ion ReproSeq™ 316 without Chips will be Q1 of 2017 and will have a 7–8 month shelf life when stocked. Based on in-stock availability, this will allow the potential to order the Ion ReproSeq™ 316 without Chips until the end of 2017. For the latest in chemistry and sequencing improvements, the supported replacement kit, the Ion ReproSeq™ PGS 316 View Kit, without chips, is now available for order.

Ion ReproSeq™ PGS 316 Kit, without chips

Catalog number: A29143

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

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The Ion ReproSeq™ PGS kits enable the fastest benchtop next-generation sequencing workflow for preimplantation aneuploidy screening.

The leading cause of in vitro fertilization (IVF) failure is the transfer of embryos with an abnormal number of chromosomes, also called aneuploidy. Preimplantation genetic screening (PGS) enables the selection of embryos with a normal number of chromosomes to help improve implantation success rates. The Ion ReproSeq PGS kits and the Ion PGM™ System allow for rapid and affordable screening of aneuploidy in all 24 chromosomes. Using as little as 6 pg of DNA from a single cell, this kit enables accurate detection of whole chromosome and chromosome arm events (>48 Mb) in as little as 8 hours*.

*Turnaround time for the Ion ReproSeq PGS workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.

Sample throughput flexibility
The Ion ReproSeq PGS kits enable the cost-effective screening of 2 to 24 samples, minimizing the need to batch embryos. By simply choosing one chip from the range of chips we provide, you can adjust your sample output to suit your needs.

Rapid turnaround for a time-sensitive procedure
Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the Ion ReproSeq PGS kits offer the fastest benchtop NGS workflow for aneuploidy screening. Go from single cells isolated from an embryo to euploid selection in as little as 8 hours when screening up to 2 samples or as little as 10 hours for up to 24 samples.

The Ion ReproSeq 316 PGS Kit includes all materials for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing up to 120 samples. The kit contains:

• Ion SingleSeq Kit (120 reactions)—allows for the extraction, amplification, and addition of barcoding of genomic DNA, a process that can be completed in less than 4 hours (includes hands on time)
• Ion PGM™ Template IA 500 Kit (8 reactions)—the fastest template preparation (2 hours, includes hands on time) whereby DNA is clonally amplified onto a bead surface through a non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ Sequencing Kit (A25592, 8 reactions at 200 bp)—offers the most advanced sequencing chemistry available and is configured to support all of your sequencing needs for applications requiring 200 bp or 400 bp read lengths
• Ion PGM™ Wash 2 Bottle Kit (A25591)—contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement

• An Ion OneTouch™ ES (enrichment system) and Ion PGM™ Enrichment Beads (4478525) are required for use with this sequencing kit.
• This kit is only compatible with the Ion PGM System.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Ion PGM™ System
Recommended Applications: Rapid Sequencing
Product Line: ReproSeq™
Product Size: 120 samples
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

• Ion PGM™ Template IA Supplies 500 (store at room temperature)
• Ion PGM™ Template IA Reagents 500 (2–8°C)
• Ion PGM™ Template IA Reactions 500, store at -30°C to -10°C
• Ion PGM™ Template IA Solutions 500 (room temperature)
• Ion SingleSeq Kit (-5 to -30°C)
• Ion PGM™ Seq Supplies (room temperature)
• Ion PGM™ Hi-Q™ Seq Reagents (-5 to -30°C)
• Ion PGM™ Hi-Q™ Seq Solutions (2–8°C)
• Ion PGM™ Hi-Q™ Seq dNTP Kit (-5 to -30°C)
• Ion PGM™ Wash 2 Bottle Kit (room temperature)


Manuals & protocols