Invitrogen™

PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit

Catalog number: A31217
Invitrogen™

PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit

Catalog number: A31217
Catalog Number
A31217
Unit Size
10 preps
Price (USD)
340.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
25 preps
Price (USD)
840.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
4 preps (sample size)
Price (USD)
138.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A3121710 preps
340.00
Your Price:
***
A3123125 preps
840.00
Your Price:
***
A330734 preps (sample size)
138.00
Your Price:
***
Product Overview
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The PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane. In as little as 90 minutes, purify up to 1.5 mg of high-quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines or genome engineering.

Advantages of the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit include:
• Simple and fast protocol—purification typically takes 90 minutes
• High yield—isolate up to 1.5 mg of high quality plasmid DNA
• Endotoxin-free plasmid—<0.1 EU/µg, ideal for sensitive applications
• High purity—ultralow levels of protein, RNA, and genomic DNA contamination
• Compatibility—suitable for use with ExpiCHO and Expi293 expression systems
• Endotoxin-free components— elution buffer, water, and plasmid storage tubes that are free of endotoxin

Simple and fast protocol
Unlike other anion-exchange-based endotoxin-free plasmid purification systems, the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit utilizes centrifugation- or vacuum-assisted filtration for lysate clarification and subsequent plasmid DNA purification. There is no waiting for gravity-flow columns and the entire purification typically takes 90 minutes (including the final precipitation step).

Endotoxin-free plasmid
Purify endotoxin-free (<0.1 EU/µg) plasmid that is ideal for sensitive applications such as transfection of primary cells, genome engineering, and research on gene therapy or plasmid vaccines in vivo.

Compatible with ExpiCHO and Expi293 expression systems
Plasmid purified using the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit is fully compatible with the Gibco ExpiCHO and Expi293 transient mammalian expression systems.

Superior quality with endotoxin-free components
Rest assured that the kit is of high quality and has additional endotoxin-free elution buffer, water, and plasmid storage tubes for added peace of mind.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Spin Column
High-throughput Compatibility
Manual Protocols
Prep Scale
> 1 mg (Large-Scale) Plasmid DNA, > 200 µg (Large-Scale) Plasmid DNA
Shipping Condition
Room Temperature
Product Line
PureLink™
For Use With (Application)
Transfection
Purification Target
Plasmid DNA
Sample Type
Bacteria
Purification Time
90 min
No. of Reactions
10 Prep
Isolation Technology
Anion Exchange Resin
Scale
Maxi

Contents & Storage

• Resuspension buffer
• Lysis indicator
• RNase A
• Lysis buffer
• Precipitation buffer
• Endotoxin removal buffer
• Wash buffer
• Elution buffer
• TE buffer
• Expi Maxi filtration column
• Expi Maxi DNA binding column
• 50 mL conical tubes

Endotoxin-free components: elution buffer, water, plasmid storage tubes

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Frequently asked questions (FAQs)

Citations & References