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Fetal Bovine Serum, qualified, Canada
Gibco™

Fetal Bovine Serum, qualified, Canada

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust ofRead more
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Catalog NumberQuantityFormat
A316070210 x 50 mLOne Shot
A316070150 mLOne Shot
12483020500 mLBottle
Our improved Value Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.
Catalog number A3160702

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

  • Sera category:Value (Standard)
  • Origin: Canada
  • Endotoxin level: ≤50 EU/mL (levels routinely ≤10 EU/mL)
  • Hemoglobin level: ≤25 mg/dL

Aliquot-free 50 mL bottle

The Gibco One Shot 50 mL bottle is designed to eliminate the need to aliquot, and the risk of contamination and variability that comes with it, improving your chances for consistent results.

Features include:

  • Wide mouth, chamfered edge, and curved shape for easy pouring and pipetting
  • Engineered to freeze/thaw without splitting or breaking
  • Fits in test tube racks and frees up freezer space
  • No labeling mishaps with our easy-to-read package label containing all of the information you need
  • 33% less waste generated than aliquoting from 500 mL bottles

All products may not be available in all regions due to importation regulations. Contact your local sales representative regarding product availability in your country.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Specifications
Product LineOne Shot
Purity or Quality GradeQualified
Shipping ConditionFrozen
SpeciesBovine
AgeFetal
Country of OriginCanada
FormatOne Shot
Product TypeFetal Bovine Serum
Quantity10 x 50 mL
Serum TreatmentStandard (Sterile-filtered)
SterilitySterile
Unit SizeEach
Contents & Storage
Storage conditions: ≤-10°C
Shipping conditions: Frozen

Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can serum be stored at 2-8 degrees C?

Serum can be kept at 2-8 degrees C for up to 4 weeks.

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Are there any other packaging configurations available for the new One Shot FBS product?

Yes, we also offer a 10 X 50 mL package, which is equivalent to a 500 mL bottle of FBS.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How is the new One Shot FBS bottle different from our existing One Shot bottle?

Our previous One Shot bottle, a 60 mL Nalgene media bottle, is not designed for easy pipetting and pouring whereas the new One Shot FBS bottle has a propietary design that was developed specifically for FBS use. This design allows you to freeze, thaw and pour without the need to aliquot, which minimizes the amount of FBS handling and reduces the risk of contamination.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, qualified, Canada, 10 x 50 mL FAQs