10-dye Spectral Calibration Kit, 96-well

The 10-dye Spectral Calibration Kit, 96-well is an easy-to-use dye calibration system to establish pure dye spectra and multicomponent values for ten fluorescent dyes: FAM, VIC, ROX, TAMRA, SYBR, ABY, JUN, MUSTANG PURPLE, NED, and Cy5. This kit is specifically engineered for use with the QuantStudio 5 Dx and QuantStudio 7 Pro Dx 96-well real-time PCR systems. The multi-dye plate format enables simultaneous calibration of multiple dyes within single plates, substantially reducing both effort and time requirements compared to calibration procedures using single dye plates. The optimized dye formulations in Plate 2 and Plate 3 enhance multiplexing performance by supporting more accurate representation of fluorescent spectra characteristic of probes utilized in real-time PCR applications.

Features of the spectral calibration kit:

• Facilitates precise wavelength accuracy and spectral response correction to enable reliable and reproducible spectroscopic measurements across the instrument's operational range
• Enhanced Multiplexing Accuracy Through Optimized Dye Formulations - the formulations of the dyes incorporated in Plate 2 and Plate 3 have been specifically engineered to improve results with multiplexing applications.
• Comprehensive Three-Plate System for Complete Spectral Coverage - The kit comprises three distinct 96-well plates, containing specific dye combinations optimized for different spectral regions.

Kit contains:

• Spectral Calibration Plate 1 (Cat. No. A31907) contains FAM, VIC, ROX, and SYBR dyes, enabling coverage for commonly used fluorophores in standard qPCR applications.
• Spectral Calibration Plate 2 (Cat. No. A31908) incorporates ABY, JUN, and MUSTANG PURPLE dyes, extending spectral coverage to specialized fluorescent reporters.
• Spectral Calibration Plate 3 (Cat. No. A31909) includes TAMRA, NED, and Cy5 dyes, completing the comprehensive spectral range necessary for advanced multiplexing applications.

Usage Guidelines and Technical Requirements

Proper use of this spectral calibration system requires following the procedures outlined in the instrument user guide to enable optimal performance, validated calibration, and compliance with quality standards. Users should consult the complete instrument documentation to achieve optimum performance and maintain compliance with established quality standards for qPCR spectral calibration procedures.