QuantStudio 5 Dx Real-Time PCR System, 96-well, 0.2 mL, desktop

Q: What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol. Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user. The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected. Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center .

QuantStudio 5 Dx Real-Time PCR System, 96-well, 0.2 mL, desktop - FAQs

View additional product information for QuantStudio 5 Dx Real-Time PCR System, 96-well, 0.2 mL, desktop - FAQs (A32006)

3 product FAQs found

Is the QuantStudio 5 Dx (IVDR) compatible with Thermo Fisher Connect?

No, the QuantStudio 5 Dx (IVDR) is not compatible with Thermo Fisher Connect.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.

The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I start a run on the QuantStudio 5 Dx with the Development Software if the instrument calibrations are expired?

No. You cannot start a run on this instrument if calibrations are expired.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.