Alexa Fluor™ 488 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.
Important Features of Alexa Fluor™ 488 Streptavidin Conjugates:
•
Alexa Fluor™ 488 streptavidin conjugate has Ex/Em maxima of ∼ (495/519)•
Bright, photostable fluorescence•
High solubility in aqueous solutions•
Available in multiple colors•
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and moreProperties of Alexa Fluor™ DyesAlexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit
improved photostability and
brightness and improved solubility in aqueous solutions. Available in a
broad range of colors, these dyes are a good choice for most imaging applications.
Blocking Endogenous BiotinNaturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our
Endogenous Biotin-Blocking Kit to minimize this interference.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links: Learn more about Avidin-Biotin DetectionLearn more about Alexa Fluor™ DyesFind out about other Labeled Streptavidin ConjugatesRead Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook
For Research Use Only. Not for use in diagnostic procedures.