Ion Torrent™

Ion Ampliseq™ Cancer Hotspot Panel v2 Chef-Ready Kit

Catalog number:  A32914

 Related applications:

Ion Torrent™ Next-Generation Sequencing

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The Ion Ampliseq™ Cancer Hotspot Panel v2 Chef-Ready Kit combines a single pool of primers with the reagents necessary to prepare libraries of frequently mutated regions in human cancer genes on the Ion Chef™ System. This automated workflow utilizes the same chemistry as the manual library construction workflow. It provides equivalent or better performance with exceptional reproducibility, on-target reads, and sample balance. Automated production of libraries using the Ion Chef System reduces hands-on time to less than 15 minutes.

The Ion Ampliseq Cancer Hostpot Panel v2 Chef Ready Kit consists of the following components:
• Ion Ampliseq™ Cancer Hotspot Research Panel Primers
• Ion Ampliseq™ Kit for Chef DL8

The simple sample-to-data workflow can be executed in less than two days and offers the following features and benefits:
• Production of high-quality DNA libraries using the Ion Chef System
• Proven performance of Ion Ampliseq™ technology, FFPE compatibility, uniform coverage for efficient sequencing, accurate variant calling, and improved variant detection via Torrent Suite™ Software
• Ability to scale and multiplex on Ion™ chips based on desired throughput needs, driving cost savings and efficiency
• Simplified workflow and reduced hands-on time when used with the Ion Ampliseq™ Direct FFPE DNA Kit
• Low input amount of only 1 ng per sample

Additional mutational content for advancement of cancer research studies
The Ion AmpliSeq Cancer Hotspot Panel v2 is designed to amplify 207 amplicons covering approximately 2,800 COSMIC mutations from 50 oncogenes and tumor suppressor genes. The panel should be used with the Ion AmpliSeq™ Library Kit 2.0.

Minimal hands-on time
Leveraging the automation capabilities of the Ion Chef System, the included Ion AmpliSeq Kit for Chef Kit DL8 enables the reproducible preparation of Ion AmpliSeq amplicon libraries, with less than 15 minutes of hands-on time and only 3 liquid transfer steps. Compatible with 1- and 2-pool Ion AmpliSeq panel designs, the kit automates the preparation of libraries from 8 samples in a single run, delivering a single tube of pooled libraries ready for downstream templating and sequencing. The kit provides sufficient material for the preparation of 32 libraries.

Simplicity and ease-of-use of Ion solutions
Utilizing plug and play, pre-packaged, single-use cartridges and PCR plates for a fully automated workflow, users of all experience levels will find library preparation straightforward with minimal effort. Supplied with barcodes conveniently dried-down in 96-well plates, multiplexing samples is easy and requires no additional pipetting steps.

For Research Use Only. Not for use in diagnostic procedures.


Library Type: Targeted Sequencing Library
Sequencing Type: DNA Sequencing
Product Line: Ion AmpliSeq™
Product Size: 32 reactions
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

• Ion Ampliseq Cancer Hotspot Panel v2 Chef Ready Kit, shipped on wet ice, store at -5 to -30°C
• 4 cartridges Ion AmpliSeq Chef Reagents DL8, shipped on wet ice, store at -5°C to -30°C
• 4 cartridges Ion AmpliSeq Chef Solutions DL8, shipped at room temperature, store at 2–8°C
• 4 boxes Ion AmpliSeq Supplies (Each box contains: Ion AmpliSeq Tip Cartridge L8, Framed PCR Foil Seal, Enrichment Cartridge), shipped at room temperature, store at room temperature
• 1 set of 4 IonCode in PCR Plates (The set includes: IonCode 0101-0108 in 96 Well PCR Plate (red), IonCode 0109-0116 in 96 Well PCR Plate (yellow), IonCode 0117-0124 in 96 Well PCR Plate (green), IonCode 0125-0132 in 96 Well PCR Plate (blue)), shipped at room temperature, store at room temperature


Manuals & protocols