Automated Thermal Cycler (ATC), 384 well, laptop, 3-m cable, 1 system - FAQs

View additional product information for Automated Thermal Cycler (ATC), 384-well, laptop - FAQs (A33978, A33977, A33979)

2 product FAQs found

Do any of your thermal cyclers, other than the Automated Thermal Cycler, hold a full skirted PCR plate?

For full skirt plate, a raised heating block is required. None of our thermal cyclers have a raised heating block except the Automated Thermal Cycler.

Find additional tips, troubleshooting help, and resources within our PCR Thermal Cyclers Support Center.

What is the optimal ramp rate for my thermal cycler?

There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.

As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.

Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf

Find additional tips, troubleshooting help, and resources within ourPCR Thermal Cyclers Support Center.