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TQMN ADV MIRNA CARD FRMT 48 - FAQs

View additional product information for TQMN ADV MIRNA CARD FRMT 48 - FAQs (A34722)

2 product FAQs found

What is a good Ct cut-off for the TaqMan MicroRNA Array Cards and TaqMan Advanced miRNA Array Cards? In other words, beyond what Ct should I not trust the data?

The typical Ct cut-off on TaqMan Array Cards is 32, which is equivalent to Ct 35 on a plate (10 µl reaction). Previous studies show that if you use pre-amplification, a Ct cut-off of 29 or 30 can be used to reduce numbers of false positives (see Technical Note Optimized protocols for human or rodent microRNA profiling with precious samples). To ensure that you have selected a correct cut-off, you should run replicates of the same sample and use Ct cut-off before you see an increase in the Standard Deviation.

What is the turnaround time for your Custom TaqMan Array MicroRNA and Advanced miRNA cards?

The turnaround time for our Custom TaqMan MicroRNA and Advanced miRNA Array cards confugurable on the website is 4-6 weeks. Our Custom-plating cards available through our Custom Services team will ship in 8-10 weeks.