Mitomycin C (MitC)-treated Gibco CF1 Mouse Embryonic Fibroblasts (MEFs) are feeder cells that are ideal for supporting healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in culture. Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. Nos. GSC-6001M and GSC-6201M, these widely-used MEF cells are isolated from outbred CF1 mice, mitotically arrested by MitC-treatment, and tested to ensure safety and performance.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Rely on a widely-used product

Help save time with ready-to-use MEFs
Irradiated CF1 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of CF1 MEFs is tested for bacteria, fungi, mycoplasma, and mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ES cells to ensure robust and consistent performance. These MEFs have successfully been used with ESC/iPSC lines from other species, but should be tested for compatibility if used for such an application.

Rely on a widely-used product
These CF1 MEFs are used in many labs worldwide and have been cited in various publications. They have been successfully used to support undifferentiated pluripotent stem cells at densities ranging from 2 x 10⁴–5.3 x 10⁴ cells/cm².