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Antibody Beacon™ Tyrosine Kinase Assay Kit

Catalog number: A35725

Molecular Probes™  Related applications: Protein Assays and Analysis | Target & Lead Identification & Validation

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Description

The Antibody Beacon Tyrosine Kinase Assay Kit provides a simple and robust assay for measuring the activity of tyrosine kinases and their inhibitors and modulators. The assay is based on a detection complex that comprises a small-molecule tracer ligand that exhibits quenched fluorescence when bound to anti-phosphotyrosine antibodies. In the presence of phosphotyrosine-containing peptides, the ligand is rapidly displaced and there is an increase in fluorescence. The Antibody Beacon tyrosine kinase assay, unlike most other commercially available tyrosine kinase activity assays, permits real-time monitoring of kinase activity.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: FRET
Assay Type: Tyrosine Kinase Assay
Product Size: 1 kit
Target Enzyme: Tyrosine Kinases
Substrate Properties: Lipid-Based Substrate
Shipping Condition: Wet Ice

Contents & storage

Store in refrigerator (2–8°C) and protect from light.

Documents

Manuals & protocols