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Pierce™ Anti-DYKDDDDK Magnetic Agarose - FAQs

View additional product information for Pierce™ Anti-DYKDDDDK Magnetic Agarose - FAQs (A36799B)

3 product FAQs found

What is the slurry/suspension percentage for your anti-DYKDDDDK ("FLAG") products?

Anti-DYKDDDDK Magnetic Agarose (Cat. Nos. A36797, A36798, A36799B) is offered as a 25% suspension (1 mL of 25% suspension = 0.25 mL of settled beads). UltraLink-based Anti-DYKDDDDK Affinity Resin (Cat. Nos, A36801, A36803, A36804) is offered as a 50% slurry (1 mL of 50% slurry = 0.5 mL of settled resin).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the binding capacity for your Anti-DYKDDDDH Magnetic Agarose?

Here is the binding capacity: ≥3.2 mg DYKDDDDK-tGFP-His protein (˜32kDa)/mL settled beads.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How do I cleave off the DYKDDDDK ("FLAG") tag after purification?

An enterokinase cleavage site behind the DYKDDDDK (“FLAG”) tag can allow complete removal of the DYKDDDDK (“FLAG”) tag leaving no additional amino acids. We offer EKMax Enterokinase (Cat. Nos. E18001 and E18002) that can be used for this purpose. Subsequently, the EKMax Enterokinase can be removed using EK-Away Resin (Cat. No. R18001), a resin conjugated with soybean trypsin inhibitor, which has high affinity for enterokinase.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.