Pierce™ Anti-DYKDDDDK Affinity Resin, 10 mL - FAQs

Anti-DYKDDDDK Magnetic Agarose (Cat. Nos. A36797, A36798, A36799B) is offered as a 25% suspension (1 mL of 25% suspension = 0.25 mL of settled beads). UltraLink-based Anti-DYKDDDDK Affinity Resin (Cat. Nos, A36801, A36803, A36804) is offered as a 50% slurry (1 mL of 50% slurry = 0.5 mL of settled resin).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Here is the binding capacity: ≥3.0 mg DYKDDDDK-tGFP-His protein (˜32 kDa)/mL settled resin.

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Yes. The UltraLink-based Anti-DYKDDDDH (“FLAG”) Affinity Resin (Cat. Nos. A36801, A36803, A36804) can be used for FPLC purification.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

An enterokinase cleavage site behind the DYKDDDDK (“FLAG”) tag can allow complete removal of the DYKDDDDK (“FLAG”) tag leaving no additional amino acids. We offer EKMax Enterokinase (Cat. Nos. E18001 and E18002) that can be used for this purpose. Subsequently, the EKMax Enterokinase can be removed using EK-Away Resin (Cat. No. R18001), a resin conjugated with soybean trypsin inhibitor, which has high affinity for enterokinase.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.