Gibco™

ExpiSf™ Protein Production Kit

Catalog number:  A3767806

 Related applications:

Insect Cell Culture

| Protein Expression

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Description

The ExpiSf Protein Production Kit is designed to significantly boost protein expression from high-density suspension cultures of Sf9 insect cells. The kit includes the chemically defined, animal origin-free ExpiSf Enhancer and ExpiSf CD Medium, which together power the highest possible level of protein expression from baculovirus-infected ExpiSf9 Cells.

The ExpiSf Protein Production Kit:
• Delivers greater than 80% more protein yield in high-density suspension Sf9 cell culture than ExpiSf CD Medium alone
• Includes ExpiSf Enhancer, which is specifically designed to work in concert with ExpiSf CD Medium to significantly boost protein expression from baculovirus-infected insect cell cultures
• Demonstrates direct scalability of baculovirus infection from culture volumes of 4 mL in deep-well plates to >3 L in shake flasks

The ExpiSf Protein Production Kit is specifically designed to work with ExpiSf9 Cells in the ExpiSf Expression System. The kit supplies sufficient ExpiSf Enhancer and ExpiSf CD Medium to infect 1 L, 10 L, or 50 L of culture. ExpiSf CD Medium is also available for purchase separately.

For Research Use or Further Manufacturing. Not for use in diagnostic procedures.

Specifications

Cell Type: Insect Cells
Classification: Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Form: Liquid
Expression: Baculovirus
Expression Mechanism: Cell-Based Expression
Product Line: ExpiSf™
Product Size: 1 L
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

• 1000 mL bottle ExpiSf CD Medium, store in the dark at 2–8°C
• 4 x 1 mL ExpiSf Enhancer, store at 2–8°C

Shelf Life: 12 months from date of manufacture

Documents

Manuals & protocols