POROS™ AAVX RoboColumn™ Pre-packed columns

RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms.

These RoboColumn™ columns are pre-packed with POROS CaptureSelect AAVX Affinity Resin (Cat. No. A36743), engineered to address the high selectivity and capacity requirements for downstream purification of a broad range of naturally occurring and synthetic adeno-associated virus (AAV) serotypes used for gene therapy applications. POROS CaptureSelect AAVX resin has demonstrated binding reactivity towards a set of AAV serotypes that includes AAV1 to AAV8 and AAVrh10, as well as synthetic serotypes.

POROS CaptureSelect AAVX resin enables a robust, fast, and platformable purification process for wide-ranging AAV serotypes with excellent purity and yield obtained in one step.

Features of POROS CaptureSelect AAVX resin include:

• One-step AAV purification from crude material with high purity and yield
• High specificity and capacity, maximizing yield by significantly reducing process steps
• Superior capacity maintained at high flow rates
• Excellent scalability and easier handling and packing due to rigid polymeric bead
• Non-animal derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

A wide selection of RoboColumn™ is available, including columns pre-packed with POROS ion exchange, HIC, and CaptureSelect affinity resins. All POROS high performance chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

RoboColumn™ is a registered trademark of Repligen Corporation.