Thermo Scientific™

Pro-Detect™ Rapid Streptag II Competitive Assay Kit

Catalog number:  A38510

 Related applications:

Protein Electrophoresis & Western Blotting

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The Thermo Scientific Pro-Detect Rapid Streptag II Competitive Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of Strep Tag II (sequence: PQKWSHPQFEK)-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to Streptag II tag to provide a qualitative detection within ten minutes. This assay can be used to detect Streptag II-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features of the Pro-Detect Rapid competitive assay kits:
Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for qualitative results within 5-10 minutes
Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
Specific—assay does not cross-react with common lysis and protein preparation reagents
Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid competitive assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. In the presence of target protein tag, the dye-bound conjugated antibodies will bind to the tagged protein present in the sample instead of the protein tag bound at the test lines. Therefore, in a positive result, a competitive lateral flow assay will show decreasing intensity of the visible test lines until all test lines have disappeared. When there is no target protein tag present, the dye-bound conjugated antibodies specific to the target will bind at the test line(s), resulting in visual test lines in a negative result.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bulk or Custom Form: Bulk Custom Form 1
Target Specificity: Target-Specific
Assay Type: Immunoassay
Detection Method: Chromogenic
Product Line: Pro-Detect™
Product Size: 10 assays
Technique: Lateral Flow Rapid Assay
Shipping Condition: Wet Ice

Contents & storage

• 10 Rapid Assay Strips
• Sample Diluent, 15 mL

Sufficient for 10 assay determinations

Store at 2–8°C.

Documents

Manuals & protocols