Collibri™ PCR-free ES DNA Library Prep Kits for Illumina Systems
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Collibri™ PCR-free ES DNA Library Prep Kits for Illumina Systems
Invitrogen™

Collibri™ PCR-free ES DNA Library Prep Kits for Illumina Systems

The Invitrogen Collibri PCR-free ES DNA Library Preparation kits are designed for next-generation sequencing (NGS) on Illumina systems. The kit supports whole-genome sequencing (WGS) applications and is suitable for all genomes.
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카탈로그 번호반응 수제품 유형
A3860202424 PrepsPCR-free ES DNA Library Prep Kit with UD Indexes (Set A, 1-24)
A38545024W24 PrepsPCR-free ES DNA Library Prep Core Kit
A38603096W96 PrepsPCR-free ES DNA Library Prep Core Kit
A3854502424 PrepsPCR-free ES DNA Library Prep Kit with CD Indexes
A3860309696 PrepsPCR-free ES DNA Library Prep Kit with CD Indexes
A4360202424 PrepsPCR-free ES DNA Library Prep Kit with UD Indexes (Set B, 25-48)
A4360302424 PrepsPCR-free ES DNA Library Prep Kit with UD Indexes (Set C, 49-72)
A4360402424 PrepsPCR-free ES DNA Library Prep Kit with UD Indexes (Set D, 73-96)
A3860819696 PrepsPCR-free ES DNA Library Prep Kit with UD Indexes (Set A-D, 1-96)
카탈로그 번호 A38602024
제품 가격(KRW)
1,475,000
온라인 행사
Ends: 31-Mar-2026
1,735,000
할인액 260,000 (15%)
Each
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반응 수:
24 Preps
제품 유형:
PCR-free ES DNA Library Prep Kit with UD Indexes (Set A, 1-24)
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
1,475,000
온라인 행사
Ends: 31-Mar-2026
1,735,000
할인액 260,000 (15%)
Each
카트에 추가하기

The Collibri PCR-free ES DNA Library Preparation Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact genomic DNA or already prepared amplicons (>300 bp) of any species. The entire library preparation protocol takes about two hours for the PCR-free workflow. If needed, Collibri Library Amplification Master Mix with primers can be bought separately. Or see the Collibri ES DNA Library Preparation Kit, designed for fast and convenient library preparation with PCR from a wide range of inputs (1–500 ng).

The Collibri PCR-free ES DNA Library Preparation Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact genomic DNA or already prepared amplicons (>300 bp) of any species. The entire library preparation protocol takes about two hours for the PCR-free workflow. If needed, Collibri Library Amplification Master Mix with primers can be bought separately. Or see the Collibri ES DNA Library Preparation Kit, designed for fast and convenient library preparation with PCR from a wide range of inputs (1–500 ng).

The Collibri PCR-free ES DNA Library Preparation Kit:
• Includes all necessary components in a single kit* for quick use
• Is empowered on miniaturization liquid handlers to cut on costs
• Provides in-process visual feedback for easier handling
•Is suitable for conventional automation to increase throughput

* The kit consists of enzymatic modification, size selection/clean-up, and indexed adapter-containing modules, except for the Core kit, which enables Collibri combination with desired indexed adapters

The Collibri PCR-free ES DNA Library Preparation Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. Moreover, the enzymatic shearing (ES) module of the kit generates a consistently high yield of library, which is enough for library quantification  and sequencing and multiple runs on Illumina instruments.

Applications
The Collibri PCR-free ES DNA Library Preparation Kit is recommended for:
Whole genome sequencing
• Resequencing
• De novo assembly

For Research Use Only. Not for use in diagnostic procedures.
사양
설명The product contains modules A38575024, A38587024, A43190024
용도(애플리케이션)Whole Genome Sequencing, Resequencing, De Novo Assembly
용도(장비)HiSeq X, HiSeq2500, HiSeq3000, HiSeq4000, MiSeq or iSeq, NextSeq 550, NovaSeq 6000
라이브러리Fragment Library
반응 수24 Preps
제품라인Collibri
제품 유형PCR-free ES DNA Library Prep Kit with UD Indexes (Set A, 1-24)
수량24 preps
샘플 종류DNA
배송 조건Dry Ice
All
워크플로우 단계Library Generation
형식Kit
Sequencing TypeGenome and DNA Sequencing
Unit SizeEach
구성 및 보관
• Collibri PCR-free ES DNA Library Prep Kit, store at -20°C
• Collibri DNA Library Cleanup Kit, store at 4°C
• Collibri DNA UD Indexes, store at -20°C

자주 묻는 질문(FAQ)

Can indexed adaptors or other modules be purchased separately for the Collibri DNA Library Prep kits?

Indexed adapters and other modules in the kit can be purchased separately. Please refer to thermofisher.com/collibriDNA and contact us for more details.

With the Collibri DNA Library Prep kits, what is the distribution of enzymatically sheared DNA in the library?

Fragment size ranges from 150 bp to 850 bp. For optimal results, we recommend and provide detailed instructions for 350 bp and 550 bp average size library construction.

With the Collibri DNA Library Prep kits, can I use indexed adaptors interchangeably between RNA-seq and DNA-seq applications?

No. They are specifically designed for a product and cannot be used interchangeably.

With the Collibri DNA Library Prep kits, why is my ligation master mix orange instead of red?

Dyes inside the master mix react with CO2 and change the color from red to orange. The mix is still usable when the color changes to orange. To prevent discoloration, avoid keeping it on dry ice.

Are Collibri DNA Library Prep kits compatible with FFPE samples?

Collibri PS DNA Library Prep Kit can be used for library construction from lower-quality DNA (such as FFPE samples). However, the yields of adaptor-ligated molecules are typically lower compared to the yields obtained with high-quality DNA.

Collibri ES DNA Library Prep Kit is not recommended for library construction from FFPE samples.