Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems, with UD indexes, 24 rxns - FAQs

Collibri Stranded RNA Library Prep Kit for Illumina Systems elution buffer is based on Tris-HCl, with no EDTA or EGTA.

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The Collibri Stranded RNA Library Prep Kit for Illumina Systems does not include an RNA control. Recommendations for RNA controls can be found in the Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina User Guide, page 16 "Guidelines for RNA Controls".

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Information for RNA sample type and amount can be found in the Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina User Guide, page 15 "Guidelines for RNA sample type and amount".

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Information regarding the different catalog numbers can be found in the Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina User Guide, page 3.
The different catalog numbers allow for the option to purchase different sizes of the library kit and with or without Unique Dual Indexes.

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A list of required materials that are not supplied with the kit can be found in the Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina User Guide, page 11-12.

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Unlike Cat. No. A38994024, Cat. No. A38996024 comes with the Unique Dual Indexes (UDIs), listed in table 6, page 10 of the Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina User Guide

Please also refer to the Technical Note "How to prevent index hopping" for additional information.

In general, UDIs have a sample index that is specific to a given library. All nucleic acids in a sample are labeled with the same sequence tag and the resulting library is pooled with other libraries and sequenced in parallel in a single run. UDIs are the preferred method when multiplexing to mitigate sample misassignment due to index hopping and the reagent kits supply enough to perform extra cycles. UDIs require extra cycles and the reagent kits supply enough to perform those extra cycles. UDIs can be especially effective when using instruments with patterned flow cells, such as the NovaSeq 6000 system.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.