Cells-to-CT™ Bulk Fast Advanced RT Reagents
Cells-to-CT™ Bulk Fast Advanced RT Reagents
Invitrogen™

Cells-to-CT™ Bulk Fast Advanced RT Reagents

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Los reactivos de RT a granel Cells-to-CT Invitrogen Fast Advanced son versiones de gran cantidad disponibles por separado de losMás información
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Número de catálogoCantidad
A391102500 reactions
Número de catálogo A39110
Precio (MXN)
-
Cantidad:
2500 reactions
Los reactivos de RT a granel Cells-to-CT Invitrogen Fast Advanced son versiones de gran cantidad disponibles por separado de los reactivos utilizados para la transcripción inversa (RT) en el kit Cells-to-CT TaqMan Fast Advanced y en el kit Cells-to-CT SYBR Green Fast Advanced (mezcla de enzimas de RT 20X y tampón de RT 20X). Estos reactivos son suficientes para 2500 reacciones de RT. Cuando se combina con el reactivo de lisis a granel Cells-to- CT, estos reactivos pueden incorporarse fácilmente en aplicaciones automatizadas de alto rendimiento.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Fidelidad (frente a Taq)20X
Características ecológicasMenos recursos utilizados y menos residuos, menos peligroso
Compatibilidad de alto rendimientoCompatible con alto rendimiento
N.º de reacciones2500 reacciones
Línea de productosCells-to-CT
Tipo de productoReactivo de Rt avanzado a granel
Diana de purificaciónLisado
Cantidad2500 reactions
Tipo de muestraCelda
Condiciones de envíoHielo seco
Suficiente para2500 reacciones
Volumen68 mL
Concentración20X
Método de detecciónSonda de cebado
Para utilizar con (aplicación)Expresión génica
GC-Rich PCR PerformanceAlto
Velocidad de reacciónFast
Unit SizeEach
Contenido y almacenamiento
• 6,8 ml de la mezcla de enzimas de RT Fast Advanced 20X
• 68 ml del tampón de RT Fast Advanced 2X

Almacenar a una temperatura de entre -5° y -30 °C.

Preguntas frecuentes

Will the Cells-to-CT system work with my special cell line?

There is no reason why the Cells-to-CT system shouldn’t work with any cell line. However, due to differences in cell size and composition, the maximum number of cells per lysis reaction may be slightly different for different cell lines. We recommend testing for inhibition and optimal cell input by using the TaqMan Cells-to-CT and SYBR Green Cells-to-CT Control kits.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What is the size of the cDNA produced by the reverse transcriptase in the Cells-to-CT Bulk Fast Advanced RT Reagents?

The reverse transcriptase in this kit can make cDNA up to 7 Kb. However, this was not specifically tested on Cells-to-Ct lysates.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

Do the 2-step Cells-to-CT kits contain RNase inhibitor?

Yes, the Stop Solution provided in the 2-step Cells-to-CT kits contains RNase inhibitor.

Can I use the TaqMan Gene Expression Cells-to-CT kit for multiplexing?

The TaqMan Gene Expression Cells-to-CT kit has been validated for duplexing. If you want to set up a multiplex real-time PCR reaction with 3 assays, we recommend using the TaqMan Fast Advanced Cells-to-CT kit (https://www.thermofisher.com/order/catalog/product/A35374).

I am using a Cells-to-CT kit and I see signal from the genomic DNA in my real-time PCR results. How do I get rid of the genomic DNA contamination?

To prevent signal from genomic DNA in the Cells-to-CT real-time PCR reaction, we recommend using a TaqMan assay or primer set that spans an exon-exon boundary, and adding DNase I to degrade genomic DNA during the lysis reaction. For optimal DNase activity in the lysis reaction, we recommend the following:
1. Ensure all media is removed from the cells.
2. Wash each well or cell pellet with an equal volume of room temperature 1X PBS.
3. Ensure the lysis reaction happens at room temperature. The lysis reaction may not reach room temperature if the plate is on ice prior to adding Lysis Solution, or cold Lysis Solution is added.
4. Warm the Lysis Solution to room temperature before adding to the cells.
5. Perform the lysis reaction at 25 degrees C for up to 8 minutes.

View all Cells-to-CT™ Bulk Fast Advanced RT Reagents FAQs