Tetracycline Hydrochloride - FAQs

Doxycycline may be used as an alternative inducing agent in the Expi293 Inducible System. It is similar to tetracycline in its mechanism of action. Doxycycline has been shown to have a longer half-life than tetracycline (48 hr vs. 24 hr, respectively). If the use of doxycycline is desired with the Expi293 Inducible System, we recommend performing a dose-response curve to determine the optimal concentration for induction.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

We recommend starting with a tetracycline concentration of 1 µg/mL to induce the expression of your protein of interest. However, you can modulate the level of expression by treating cells with different amounts of tetracycline. We recommend performing a dose-response curve (0-1 µg/mL; 2-fold dilution) to determine the optimal concentration of tetracycline required to induce your protein of interest.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.