Gibco™

Expi293™ Inducible Expression System Kit

Catalog number:  A39251

 Related applications:

Mammalian Cell Culture

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Description

The Expi293 Inducible Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered Expi293F cells that stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression. The Expi293F Inducible cell line was established by the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter. This cell line is a powerful tool for tetracycline-inducible high-yield expression of recombinant proteins.

Expi293 Inducible Expression System Kit features include:
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
• Suspension, high-density culture of Expi293F Inducible cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

The components included in the Expi293 Inducible Expression System Kit are: one vial of frozen Expi293F Inducible cells, 1 L of Expi293 Expression Medium, one ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture, 100 mL Opti-Plex Complexation Buffer, one pcDNA5/TO Mammalian Expression Vector kit, 500 mg tetracycline hydrochloride, and the pRABBIT IgG IRES-EmGFP positive control vector. All components of the Expi293 Inducible Expression System are also available for individual purchase.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Line: Expi293F™
Delivery Method: Transfection
Key Function: Protein Production
Product Line: Expi293™
Product Size: 1 kit
Promoter: CMV⁄TO
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice

Contents & storage

• Expi293F Inducible Cells (1x107 cells/vial), store in vapor-phase liquid nitrogen
• 1 L Expi293 Expression Medium, store at 2–8°C (protect from light)
• ExpiFectamine 293 Transfection Kit for 1 L of culture, store at 2–8°C
• 100 mL Opti-Plex Complexation Buffer, store at 2–8°C (protect from light)
• 20 µg pcDNA5/TO Mammalian Expression Vector, store at 2–8°C or long term storage at -20°C.
• pRABBIT IgG IRES-EmGFP Positive Control Vector, store at 2–8°C or long term storage at -20°C.
• 500 mg Tetracycline Hydrochloride, store at -20°C

Documents

Manuals & protocols