Thermo Scientific™

SureQuant™ AKT Pathway (Phospho) IP and MS Sample Preparation Module

Catalog number:  A40086

 Related applications:

Protein Mass Spectrometry Analysis

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Description

The Thermo Scientific SureQuant AKT Pathway (Phospho) IP and MS Sample Preparation Module provides a complete reagent solution for the multiplexed enrichment and sample preparation for studies of key phosphorylation events of the AKT signaling pathway using mass spectrometry (MS).

SureQuant AKT Pathway (Phospho) IP and MS Sample Preparation Module features include:
• Complete—includes all of the reagents necessary for sample lysis, immuno-enrichment, and MS sample preparation
• Validated—antibodies validated for specificity and background
• Optimized—procedure simplified and condensed to prepare samples after immunoprecipitation in four hours
• Multiplex—able to immuno-enrich phosphorylated AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2 in a single reaction

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and phosphorylation plays a major role in its modulation. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This module is ideal for the multiplexed immuno-enrichment of key phosphorylated proteins in the AKT signaling pathway and has been optimized for use with the SureQuant quantitation modules.

The SureQuant AKT Pathway (Phospho) IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, with non-specific proteins removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

This kit can be used with the either the SureQuant AKT Pathway (Phospho) Absolute Quantitation Module or the SureQuant AKT Pathway (Phospho) Relative Quantitation Module for protein targeted quantitation. When used together, these modules provide a complete solution for sample preparation and analysis of the phosphorylated target proteins in the pathway.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Mass Spectrometry
Final Product: Peptide
For Use With (Equipment): Mass Spectrometer, Thermo Scientific™ Q Exactive Mass Spectrometer
Product Size: 10 reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice
Starting Material: Protein samples
Workflow Step: Target Selection

Documents

Manuals & protocols