Thermo Scientific™

SureQuant™ RAS Isoform IP and MS Sample Preparation Module

Catalog number:  A40096

 Related applications:

Protein Mass Spectrometry Analysis

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Description

The Thermo Scientific SureQuant RAS Isoform IP and MS Sample Preparation Module provides a complete reagent solution for the multiplexed enrichment and sample preparation of HRAS, NRAS, and KRAS for mass spectrometry (MS).

SureQuant RAS Isoform IP and MS Sample Preparation Module features include:
Complete—includes all of the reagents necessary for sample lysis, immuno-enrichment, and MS sample preparation
Validated—antibodies validated for specificity and background
Optimized—procedure simplified and condensed to prepare samples after immunoprecipitation in four hours
Multiplex—able to immuno-enrich HRAS, NRAS, and KRAS isoforms in a single reaction

The RAS family of GTPase proteins modulates two key signaling pathways involved in cell growth and proliferation: the MAPK and PI3K pathways. Aberrant activation of the RAS proteins promotes constitutive activation of cell growth and proliferation that can result in cancer. Mutations in the NRAS, KRAS, and HRAS oncogenes are the most common mutations in human cancer. This module is ideal for multiplexed immuno-enrichment of RAS isoforms for use with the SureQuant quantitation modules.

The SureQuant RAS Pathway IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, where non-specific proteins are removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

This kit can be used with the either the SureQuant RAS Isoform Absolute Quantitation Module or the SureQuant RAS Isoform Relative Quantitation Module for protein targeted quantitation. When used together, these modules provide a complete solution for sample preparation and analysis of the target proteins.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Mass Spectrometry
Final Product: Peptide
For Use With (Equipment): Mass Spectrometer, Thermo Scientific™ Q Exactive Mass Spectrometer
Product Size: 10 reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice
Starting Material: Protein samples
Workflow Step: Target Selection

Documents

Manuals & protocols