Thermo Scientific™

SureQuant™ TP53 Panel IP and MS Sample Preparation Module

Catalog number:  A40101

 Related applications:

Protein Mass Spectrometry Analysis

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Description

The Thermo Scientific SureQuant TP53 Panel IP and MS Sample Preparation Module provides a complete reagent solution for the multiplexed enrichment and sample preparation of TP53 for mass spectrometry (MS).

SureQuant TP53 Isoform IP and MS Sample Preparation Module features include:
Complete—includes all of the reagents necessary for sample lysis, immuno-enrichment, and MS sample preparation
Validated—antibodies validated for specificity and background
Optimized—procedure simplified and condensed to prepare samples after immunoprecipitation in four hours
Multiplex—able to immuno-enrich TP53 and phosphorylated TP53 protein

The tumor suppressor protein TP53 functions as a negative checkpoint regulator for abnormal cell signaling and can induce cell cycle arrest, activate DNA repair proteins, or initiate apoptosis. Mutations in TP53 often result abnormal cell growth and are commonly found in many types of cancer. This module is ideal for multiplexed immuno-enrichment of TP53 protein for use with the SureQuant quantitation modules.

The SureQuant TP53 Panel IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, where non-specific proteins and removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

This kit can be used with the either the SureQuant TP53 Panel Absolute Quantitation Module or the SureQuant TP53 Panel Relative Quantitation Module for protein targeted quantitation. When used together, these modules provide a complete solution for sample preparation and analysis of the target proteins.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Mass Spectrometry
Final Product: Peptide
For Use With (Equipment): Mass Spectrometer, Thermo Scientific™ Q Exactive Mass Spectrometer
Product Size: 10 reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice
Starting Material: Protein samples
Workflow Step: Target Selection

Documents

Manuals & protocols