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Ion 540™ Kit for Agrigenomics—Chef - FAQs

Ion 540™ Kit for Agrigenomics—Chef - FAQs

View additional product information for Ion 540™ Kit for Agrigenomics—Chef - FAQs (A43541)

10 product FAQs found

Is Ion S5 Sequencing Reagents (Part No. A27768) used for both the Ion 540 and Ion 550 Chip sequencing?

Yes, Ion S5 Sequencing Reagents (Part No. A27768) is used on the instrument for both Ion 540 and Ion 550 Chip sequencing.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center

Can I use the Ion 540 Kit - Chef to prepare samples from the Ion 16S Metagenomics Kit?

No, the Ion 16S Metagenomics Kit utilizes 400 bp reads, so it is incompatible with the Ion 540 Kit - Chef, which only yields 200 bp reads.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What version of Torrent Suite Software is compatible with the Ion 540 Kit - Chef?

The Ion 540 Kit - Chef is compatible with Torrent Suite Software v5.0 and above.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How long is the Ion Chef run for the Ion 540 Kit - Chef?

Two Ion 540 chips can be prepped and loaded in 13 hours, with only 15 minutes of hands on time.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many reads can I get with the Ion 540 Kit - Chef?

The Ion 540 Kit - Chef can yield up to 80 M reads.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I perform 400 bp sequencing with the Ion 540 Kit - Chef?

No, the Ion 540 Kit - Chef can only perform 200 bp sequencing runs.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How long is the initialization stable for the Ion 540 Kit - Chef?

The initialization is stable for 24 hours. Both runs, if performing two 20 0bp runs, must be started within 24 hours of the end of the initialization.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many runs can I perform with the Ion 540 Kit - Chef?

The Ion 540 Kit - Chef (Cat. No. A27759) contains reagents and consumables for performing eight 200 bp runs (1 run per initialization). The Ion 540 Kit - Chef (Cat. No. A30011) contains reagents and consumables for eight 200 bp runs (2 runs per initialization).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the shelf life of the Ion 540 Kit - Chef?

The shelf life of the Ion 540 Kit - Chef is 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the components of the Ion 540 Kit - Chef?

The components of the Ion 540 Kit - Chef can be found here (https://tools.thermofisher.com/content/sfs/manuals/MAN0010848_Ion540_Chef_PI.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.