NGS Reverse Transcription Kit

The Ion Torrent NGS Reverse Transcription (RT) Kit is the first cDNA synthesis kit developed specifically for next-generation sequencing (NGS) applications. It combines the superior performance of SuperScript IV Reverse Transcriptase with a novel master mix optimized for NGS library preparation. The NGS RT Kit is compatible with Ion AmpliSeq and Ion AmpliSeq HD assays and can be used with both manual and automated workflows.

Benefits of the NGS RT Kit include:
• Optimized for a variety of sample types, including FFPE, degraded samples, and those with low template amounts
• Superior NGS results with novel RT formulation optimized for NGS library preparation
• Helps increase NGS library yield, improve detection of long amplicons, and rescue under-performing amplicons
• Helps maximize mapped reads and improve detection of RNA fusions
• Trusted SuperScript IV RT technology offers superior cDNA synthesis performance with even the most challenging RNA samples
• Universal kit for wide variety of Oncomine oncology research assays and Ion AmpliSeq panels

Simple reaction setup
The NGS RT Kit contains a tube of 10X SuperScript IV enzyme mix and a tube of 5X buffer. A one-step, 25-minute reaction reverse transcribes total RNA into cDNA and can be used directly for NGS library preparation, without any additional quantification, purification, or dilution steps. A variety of sample types can be used, including RNA from blood, bone marrow, FFPE and partially degraded samples. This product is compatible with Oncomine and Ion AmpliSeq workflows, as well as Ion AmpliSeq HD panels. Consult the cDNA synthesis section of each assay manual for specific RT reaction volumes and setup instructions.

Performance and quality testing
The NGS RT Kit is assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product. It is QC tested with a full NGS workflow to evaluate library yield and ensure performance meets key sequencing metrics.